OXA-372, a novel carbapenem-hydrolysing class D β-lactamase from a Citrobacter freundii isolated from a hospital wastewater plant

Antonelli, A.; D'Andrea, M. M.; Vaggelli, G.; Docquier, Jean-Denis; Rossolini, G. M.

doi:10.1093/jac/dkv181

Article (Scientific journals)

OXA-372, a novel carbapenem-hydrolysing class D β-lactamase from a Citrobacter freundii isolated from a hospital wastewater plant

Antonelli, A.; D'Andrea, M. M.; Vaggelli, G. et al.

2015 • In Journal of Antimicrobial Chemotherapy, 70 (10), p. 2749-2756

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/252806

DOI
10.1093/jac/dkv181

PubMed
26126492

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Keywords :

Article; Citrobacter freundii; Escherichia coli; Italy; DNA sequence; Amino Acid Sequence; Anti-Bacterial Agents; Bacterial Proteins; Carbapenems; Cloning, Molecular; Humans; Microbial Sensitivity Tests; Molecular Sequence Data; Phylogeny; Sequence Alignment; Sequence Analysis, DNA; Waste Water

Abstract :

[en] Objectives: The objective of this study was to characterize a novel class D carbapenemase, named OXA-372, identified in a carbapenem-resistant Citrobacter freundii strain (Cfr-FI-07) isolated from a hospital wastewater plant in central Italy. Methods: Cfr-FI-07 was isolated using a selective chromogenic medium for carbapenem-resistant Enterobacteriaceae. Carbapenemase production was confirmed by spectrophotometric assay. WGS was carried out using an Illumina MiSeq platform. The functional profile of OXA-372 was investigated by expression of the cloned gene in Escherichia coli and by analysis of kinetic parameters of the purified enzyme. Results: C. freundii Cfr-FI-07 produced carbapenemase activity, but tested negative for common carbapenemase genes. WGS confirmed the absence of known carbapenemase genes and revealed the presence of a novel class D b-lactamase (DBL) determinant, named blaOXA-372, encoding a protein distantly related to other DBLs. In E. coli, production of OXA-372 conferred resistance to penicillins, including temocillin, and reduced susceptibility to carbapenems, while susceptibility to expanded-spectrum cephalosporins was virtually unaffected. This substrate specificity was confirmed by kinetic characterization of the purified enzyme, which exhibited high catalytic efficiencies for carbapenems (kcat/KM values ≥0.22 s2-1.μM-1). The blaOXA-372 genewas associated with a genetic platform of original structure consisting of a Tn402/Tn5053 hybrid transposon derivative, named Tn6255, inserted into a TnPa38-like transposon, named Tn6256, located on an IncA/C-IncN plasmid of approximately 140 kb. Conclusions: OXA-372 is a novel class D carbapenemase, belonging to a new lineage of DBLs, encoded by a gene associated with mobile elements. Functional properties revealed similarities, but also some differences, compared with other class D carbapenemases. © The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.

Disciplines :

Biochemistry, biophysics & molecular biology
Microbiology

Author, co-author :

Antonelli, A.; Department of Medical Biotechnology, University of Siena, Siena, Italy, Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy

D'Andrea, M. M.; Department of Medical Biotechnology, University of Siena, Siena, Italy

Vaggelli, G.; Clinical Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy

Docquier, Jean-Denis ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines

Rossolini, G. M.; Department of Medical Biotechnology, University of Siena, Siena, Italy, Clinical Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy

Language :

English

Title :

OXA-372, a novel carbapenem-hydrolysing class D β-lactamase from a Citrobacter freundii isolated from a hospital wastewater plant

Publication date :

2015

Journal title :

Journal of Antimicrobial Chemotherapy

ISSN :

0305-7453

eISSN :

1460-2091

Publisher :

Oxford University Press

Volume :

Issue :

Pages :

2749-2756

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 19 November 2020

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