Biochemical characterization of VIM-39, a VIM-1-like metallo-β-lactamase variant from a multidrug-resistant Klebsiella pneumoniae isolate from Greece

Papagiannitsis, C. C.; Pollini, S.; De Luca, F.; Rossolini, G. M.; Docquier, Jean-Denis; Hrabák, J.

doi:10.1128/AAC.01935-15

Article (Scientific journals)

Biochemical characterization of VIM-39, a VIM-1-like metallo-β-lactamase variant from a multidrug-resistant Klebsiella pneumoniae isolate from Greece

Papagiannitsis, C. C.; Pollini, S.; De Luca, F. et al.

2015 • In Antimicrobial Agents and Chemotherapy, 59 (12), p. 7811-7814

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/252805

DOI
10.1128/AAC.01935-15

PubMed
26369975

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Keywords :

VIM-1 metallo-beta-lactamase; Article; Escherichia coli; Greece; Klebsiella pneumoniae; DNA sequence; Klebsiella Infections; Ampicillin; Anti-Bacterial Agents; Bacterial Typing Techniques; Base Sequence; Biotransformation; Cephalothin; Gene Expression; Humans; Hydrolysis; Imipenem; Isoenzymes; Kinetics; Microbial Sensitivity Tests; Molecular Sequence Data; Sequence Analysis, DNA

Abstract :

[en] VIM-39, a VIM-1-like metallo-β-lactamase variant (VIM-1 Thr33Ala His224Leu) was identified in a clinical isolate of Klebsiella pneumoniae belonging to sequence type 147. VIM-39 hydrolyzed ampicillin, cephalothin, and imipenem more efficiently than did VIM-1 and VIM-26 (a VIM-1 variant with the His224Leu substitution) because of higher turnover rates. © 2015 American Society for Microbiology. All Rights Reserved.

Disciplines :

Biochemistry, biophysics & molecular biology
Microbiology

Author, co-author :

Papagiannitsis, C. C.; Faculty of Medicine, University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic, Biomedical Center, Faculty of Medicine in Plzen, Charles University in Prague, Plzen, Czech Republic

Pollini, S.; Department of Medical Biotechnologies, University of Siena, Siena, Italy

De Luca, F.; Department of Medical Biotechnologies, University of Siena, Siena, Italy

Rossolini, G. M.; Department of Medical Biotechnologies, University of Siena, Siena, Italy, Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy, Clinical Microbiology and Virology Unit, Careggi University Hospital, Florence, Italy

Docquier, Jean-Denis ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines

Hrabák, J.; Faculty of Medicine, University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic, Biomedical Center, Faculty of Medicine in Plzen, Charles University in Prague, Plzen, Czech Republic

Language :

English

Title :

Biochemical characterization of VIM-39, a VIM-1-like metallo-β-lactamase variant from a multidrug-resistant Klebsiella pneumoniae isolate from Greece

Publication date :

2015

Journal title :

Antimicrobial Agents and Chemotherapy

ISSN :

0066-4804

eISSN :

1098-6596

Publisher :

American Society for Microbiology

Volume :

Issue :

Pages :

7811-7814

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

Grants NT11032-6/2010 and 15-28663A; Charles University Research Fund (project number P36); National Sustainability Program I (NPU I) Nr. LO1503

Funding text :

We are very thankful to Marek Gniadkowski for providing the VIM producing K. pneumoniae isolates used in the present study. This work was supported by grants NT11032-6/2010 and 15-28663A from the Ministry of Health of the Czech Republic, by the Charles University Research Fund (project number P36), and by the National Sustainability Program I (NPU I) Nr. LO1503 provided by the Ministry of Education, Youth and Sports of the Czech Republic. We have no conflict of interest to declare.

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