Article (Scientific journals)
Biochemical characterization of CPS-1, a subclass B3 metallo-β-lactamase from a Chryseobacterium piscium soil isolate
Gudeta, D. D.; Pollini, S.; Docquier, Jean-Denis et al.
2016In Antimicrobial Agents and Chemotherapy, 60 (3), p. 1869-1873
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Keywords :
Article; Bradyrhizobium japonicum; Chryseobacterium; Chryseobacterium formosense; Chryseobacterium piscium; Elizabethkingia meningoseptica; Escherichia coli; Legionella; Legionella gormanii; Pseudomonas aeruginosa; Serratia marcescens; Stenotrophomonas maltophilia; Amino Acid Sequence; Anti-Bacterial Agents; Carbapenems; Cephalosporins; Cloning, Molecular; DNA, Bacterial; Drug Resistance, Multiple, Bacterial; Microbial Sensitivity Tests; Penicillins; Sequence Alignment; Soil Microbiology
Abstract :
[en] CPS-1 is a subclass B3 metallo-β-lactamase from a Chryseobacterium piscium isolate collected from soil, showing 68% amino acid identity to the GOB-1 enzyme. CPS-1 was overproduced in Escherichia coli Rosetta (DE3), purified by chromatography, and biochemically characterized. This enzyme exhibits a broad-spectrum substrate profile, including penicillins, cephalosporins, and carbapenems, which overall resembles those of L1, GOB-1, and acquired subclass B3 enzymes AIM-1 and SMB-1. Copyright © 2016, American Society for Microbiology. All Rights Reserved.
Disciplines :
Biochemistry, biophysics & molecular biology
Microbiology
Author, co-author :
Gudeta, D. D.;  Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark
Pollini, S.;  Department of Medical Biotechnologies, University of Siena, Siena, Italy
Docquier, Jean-Denis ;  Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines
Bortolaia, V.;  Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark
Rossolini, G. M.;  Department of Medical Biotechnologies, University of Siena, Siena, Italy, Department of Experimental, Clinical Medicine, University of Florence, Florence, Italy, Clinical Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy
Guardabassi, L.;  Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark, Department of Biomedical Sciences, Ross University, School of Veterinary Medicine, St. Kitts, Trinidad and Tobago
Language :
English
Title :
Biochemical characterization of CPS-1, a subclass B3 metallo-β-lactamase from a Chryseobacterium piscium soil isolate
Publication date :
2016
Journal title :
Antimicrobial Agents and Chemotherapy
ISSN :
0066-4804
eISSN :
1098-6596
Publisher :
American Society for Microbiology
Volume :
60
Issue :
3
Pages :
1869-1873
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 November 2020

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