Suppressive Effects of Barley β-Glucans with Different Molecular Weight on 3T3-L1 Adipocyte Differentiation

Barley β-glucans; C/EBPα; PPARγ; CCAAT enhancer binding protein alpha; Ucp2 protein, mouse; Hordeum; Adipocytes; Adipogenesis; Adipose Tissue; Animals; CCAAT-Enhancer-Binding Protein-alpha; Cell Differentiation; Down-Regulation; Glucose Transporter Type 4; Ion Channels; Lipoprotein Lipase; Mice; Mitochondrial Proteins; Molecular Weight; Obesity; Plant Extracts; PPAR gamma; RNA, Messenger; Triglycerides; Uncoupling Protein 2

Abstract :

[en] In this study, 2 β-glucans with different molecular weight were prepared and purified from hull-less barley bran. The aim was to evaluate their effects on the differentiation of 3T3-L1 pre-adipocytes. Results showed that barley β-glucans inhibited the differentiation of 3T3-L1 pre-adipocytes induced by differentiation medium in a dose-dependent manner, the suppressive effect of high-molecular-weight barley β-glucans (552 kDa, BGH) was stronger (P < 0.05) than that of low-molecular-weight barley β-glucan (32 kDa, BGL), evidenced by the significantly decrease (P < 0.05) of Oil-red O staining and intracellular triglyceride content in the mature adipocytes. Besides, gene expression analysis and Western Blot analysis revealed that both BGH and BGL inhibited the mRNA and protein levels of adipogenesis related transcription factors peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT-enhancer-binding protein α (C/EBPα) which are principal regulators of adipogenesis. Furthermore, the mRNA and protein expression levels of PPARγ target genes in adipose tissue including adipocyte fatty acid binding protein (ap2), lipoprotein lipase (LPL), uncoupling protein-2 (UCP-2), and glucose-transporter 4 (Glut4) in 3T3-L1 cells was also markedly downregulated (P < 0.05). These findings were anticipated to help develop barley β-glucans based functional food for the management of obesity. © 2016 Institute of Food Technologists®.

Disciplines :

Agriculture & agronomy

Author, co-author :

Zhu, Yingying ; Université de Liège - ULiège > Doct. sc. agro. & ingé. biol. (Paysage)

Yao, Y.; Inst. of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China

Gao, Y.; Inst. of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China, Qilu Univ. of Technology, Daxue Road, Western Univ, Science Park, Jinan, Shandong, China

Hu, Y.; Inst. of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China

Shi, Z.; Inst. of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China

Ren, G.; Inst. of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China

Language :

English

Title :

Suppressive Effects of Barley β-Glucans with Different Molecular Weight on 3T3-L1 Adipocyte Differentiation

Publication date :

2016

Journal title :

Journal of Food Science

ISSN :

0022-1147

eISSN :

1750-3841

Publisher :

Wiley

Volume :

Issue :

Pages :

H786-H793

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

Hong Kong, Macao, and Taiwan Science and Technology Cooperation Program of China (2013DFH30050); Special Fund for Agro-scientific Research in the Public Interest (201403063); Earmarked Fund for China Agriculture Research System Modern Agro-313 industry Technology (CYTX-014); Agricultural Science and Technology Program for Innovation Team on Identification and excavation of Elite Crop Germplasm, CAAS

Funders :

CAAS - Chinese Academy of Agricultural Sciences

Available on ORBi :

since 07 November 2020

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Bibliography

Alminger M, Eklund-Jonsson C. 2008. Whole-grain cereal products based on a high-fibre barley or oat genotype lower post-prandial glucose and insulin responses in healthy humans. Eur J Nutr 47:294-300.
Artiss JD, Brogan K, Brucal M, Moghaddam M, Jen KLC. 2006. The effects of a new soluble dietary fiber on weight gain and selected blood parameters in rats. Metabolism 55:195-202.
Aubert J, Champigny O, Saint-Marc P, Negrel R, Collins S, Ricquier D, Ailhaud G. 1997. Up-regulation of UCP-2 gene expression by PPAR agonists in preadipose and adipose cells. Biochem Biophys Res Commun 238:606-11.
Bae IY, Lee S, Kim SM, Lee HG. 2009. Effect of partially hydrolyzed oat β-glucan on the weight gain and lipid profile of mice. Food Hydrocoll 23:2016-21.
Beer MU, Wood PJ, Weisz J. 1997. Molecular weight distribution and (1→ 3) (1→ 4)-β-D-glucan content of consecutive extracts of various oat and barley cultivars 1. Cereal Chem 74:476-80.
Biörklund M, Van Rees A, Mensink RP, Önning G. 2005. Changes in serum lipids and postprandial glucose and insulin concentrations after consumption of beverages with β-glucans from oats or barley: a randomised dose-controlled trial. Eur J Clin Nutr 59:1272-81.
Cani PD, Bibiloni R, Knauf C, Waget A, Neyrinck AM, Delzenne NM, Burcelin R. 2008. Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice. Diabetes 57:1470-81.
Darwiche G, Björgell O, Almér LO. 2003. The addition of locust bean gum but not water delayed the gastric emptying rate of a nutrient semisolid meal in healthy subjects. BMC Gastroenterol 3:12.
Eastwood MA, Morris ER. 1992. Physical properties of dietary fiber that influence physiological function: a model for polymers along the gastrointestinal tract. Am J Clin Nutr 55:436-42.
Edwards CA, Johnson IT, Read NW. 1988. Do viscous polysaccharides slow absorption by inhibiting diffusion or convection? Eur J Clin Nutr 42:307-12.
Farmer SR. 2006. Transcriptional control of adipocyte formation. Cell Metab 4:263-73.
Gustafson B, Jack MM, Cushman SW, Smith U. 2003. Adiponectin gene activation by thiazolidinediones requires PPARγ2, but not C/EBPα-evidence for differential regulation of the aP2 and adiponectin genes. Biochem Biophys Res Commun 308:933-9.
Kawai M, Namba N, Mushiake S, Etani Y, Nishimura R, Makishima M, Ozono K. 2007. Growth hormone stimulates adipogenesis of 3T3-L1 cells through activation of the Stat5A/5B-PPARγ pathway. J Mol Endocrinol 38:19-34.
Kanagasabapathy G, Chua KH, Malek SNA, Vikineswary S, Kuppusamy UR. 2014. AMP-activated protein kinase mediates insulin-like and lipo-mobilising effects of β-glucan-rich polysaccharides isolated from Pleurotus sajor-caju (Fr.), Singer mushroom, in 3T3-L1 cells. Food chem 145:198-204.
Kersten S, Desvergne B, Wahli W. 2000. Roles of PPARs in health and disease. Nature 405:421-4.
Kowalska K, Olejnik A, Rychlik J, Grajek W. 2014. Cranberries (Oxycoccus quadripetalus) inhibit adipogenesis and lipogenesis in 3T3-L1 cells. Food Chem 148:246-52.
Knuckles BE, Chiu MCM. 1999. β-Glucanase activity and molecular weight of β-glucans in barley after various treatments. Cereal Chem 76:92-5.
Lee IS, Kim JP, Ryoo IJ, Kim YH, Choo SJ, Yoo ID. 2010. Lanostane triterpenes from Ganoderma lucidumsuppress the adipogenesis in 3T3-L1 cells through down-regulation of SREBP-1c. Bioorg Med Chem Lett 20:5577-81.
Liu Q, Xu X, Zhang L, Yu J. 2012. Interaction between polydeoxyadenylic acid and β-glucan from Lentinus edodes. Eur Polym J 48:1329-38.
Mark DH. 2005. Deaths attributable to obesity. J Am Vet Med Assoc 293:1918-9.
Newman RK, Newman CW. 1991. Barley as a food grain. Cereal Food World 36, 800-5.
Nicolosi R, Bell SJ, Bistrian BR, Greenberg I, Forse RA, Blackburn GL. 1999. Plasma lipid changes after supplementation with β-glucan fiber from yeast. AM J Clin Nutr 70:208-12.
Noh JR, Kim YH, Hwang JH, Gang GT, Yeo SH, Kim KS, Lee CH. 2013. Scoparone inhibits adipocyte differentiation through down-regulation of peroxisome proliferators-activated receptor γ in 3T3-L1 preadipocytes. Food Chem 141:723-30.
Rosen ED, Sarraf P, Troy AE, Bradwin G, Moore K, Milstone DS, Spiegelman BM, Mortensen RM. 1999. PPARγ is required for the differentiation of adipose tissue in vivo and in vitro. Mol Cell 4:611-7.
Rosen ED, Walkey CJ, Puigserver P, Spiegelman BM. 2000. Transcriptional regulation of adipogenesis. Gene Dev 14:1293-307.
Schneeman BO, Gallaher D. 1985. Effects of dietary fiber on digestive enzyme activity and bile acids in the small intestine. Exp Biol Med 180:409-14.
Shen RL, Dang XY, Dong JL, Hu XZ. 2012. Effects of oat β-glucan and barley β-glucan on fecal characteristics, intestinal microflora, and intestinal bacterial metabolites in rats. J Agric Food Chem 60:11301-8.
Smith KN, Queenan KM, Thomas W, Fulcher RG, Slavin JL. 2008. Physiological effects of concentrated barley β-glucan in mildly hypercholesterolemic adults. J AM Coll Nutr 27:434-40.
Vaidya H, Goyal RK, Cheema SK. 2012. Anti-diabetic activity of swertiamarin is due to an active metabolite, gentianine, that upregulates PPAR-γ gene expression in 3T3-L1 cells. Cell 40:60.
Weickert MO, Pfeiffer AF. 2008. Metabolic effects of dietary fiber consumption and prevention of diabetes. J Nutr 138:439-42.
Xu C, Lv J, Lo YM, Cui SW, Hu X, Fan M. 2013. Effects of oat β-glucan on endurance exercise and its anti-fatigue properties in trained rats. Carbohyd Polym 92:1159-65.
Yim MJ, Hosokawa M, Mizushina Y, Yoshida H, Saito Y, Miyashita K. 2011. Suppressive effects of amarouciaxanthin A on 3T3-L1 adipocyte differentiation through down-regulation of PPARγ and C/EBPα mRNA expression. J Agric Food chem 59:1646-52.
Zheng X, Li L, Wang Q. 2011. Distribution and molecular characterization of β-glucans from hull-less barley bran, shorts and flour. Intl J Mol Sci 12:1563-74.