Suppressive Effects of Barley β-Glucans with Different Molecular Weight on 3T3-L1 Adipocyte Differentiation

Barley β-glucans; C/EBPα; PPARγ; CCAAT enhancer binding protein alpha; Ucp2 protein, mouse; Hordeum; Adipocytes; Adipogenesis; Adipose Tissue; Animals; CCAAT-Enhancer-Binding Protein-alpha; Cell Differentiation; Down-Regulation; Glucose Transporter Type 4; Ion Channels; Lipoprotein Lipase; Mice; Mitochondrial Proteins; Molecular Weight; Obesity; Plant Extracts; PPAR gamma; RNA, Messenger; Triglycerides; Uncoupling Protein 2

Abstract :

[en] In this study, 2 β-glucans with different molecular weight were prepared and purified from hull-less barley bran. The aim was to evaluate their effects on the differentiation of 3T3-L1 pre-adipocytes. Results showed that barley β-glucans inhibited the differentiation of 3T3-L1 pre-adipocytes induced by differentiation medium in a dose-dependent manner, the suppressive effect of high-molecular-weight barley β-glucans (552 kDa, BGH) was stronger (P < 0.05) than that of low-molecular-weight barley β-glucan (32 kDa, BGL), evidenced by the significantly decrease (P < 0.05) of Oil-red O staining and intracellular triglyceride content in the mature adipocytes. Besides, gene expression analysis and Western Blot analysis revealed that both BGH and BGL inhibited the mRNA and protein levels of adipogenesis related transcription factors peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT-enhancer-binding protein α (C/EBPα) which are principal regulators of adipogenesis. Furthermore, the mRNA and protein expression levels of PPARγ target genes in adipose tissue including adipocyte fatty acid binding protein (ap2), lipoprotein lipase (LPL), uncoupling protein-2 (UCP-2), and glucose-transporter 4 (Glut4) in 3T3-L1 cells was also markedly downregulated (P < 0.05). These findings were anticipated to help develop barley β-glucans based functional food for the management of obesity. © 2016 Institute of Food Technologists®.

Disciplines :

Agriculture & agronomy

Author, co-author :

Zhu, Yingying ; Université de Liège - ULiège > Doct. sc. agro. & ingé. biol. (Paysage)

Yao, Y.; Inst. of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China

Gao, Y.; Inst. of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China, Qilu Univ. of Technology, Daxue Road, Western Univ, Science Park, Jinan, Shandong, China

Hu, Y.; Inst. of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China

Shi, Z.; Inst. of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China

Ren, G.; Inst. of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China

Language :

English

Title :

Suppressive Effects of Barley β-Glucans with Different Molecular Weight on 3T3-L1 Adipocyte Differentiation

Publication date :

2016

Journal title :

Journal of Food Science

ISSN :

0022-1147

eISSN :

1750-3841

Publisher :

Wiley

Volume :

Issue :

Pages :

H786-H793

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

Hong Kong, Macao, and Taiwan Science and Technology Cooperation Program of China (2013DFH30050); Special Fund for Agro-scientific Research in the Public Interest (201403063); Earmarked Fund for China Agriculture Research System Modern Agro-313 industry Technology (CYTX-014); Agricultural Science and Technology Program for Innovation Team on Identification and excavation of Elite Crop Germplasm, CAAS

Funders :

CAAS - Chinese Academy of Agricultural Sciences [CN]

Available on ORBi :

since 07 November 2020

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