Immunoregulatory activities of polysaccharides from mung bean

Mung bean; Ultrasonic; Water-extractable polysaccharide; Gel permeation chromatography; Macrophages; Ultrasonic equipment; Ultrasonics; Water treatment; Anion exchange; Average molecular weight; Gel-filtration chromatography; In-vitro; Mungbeans; Murine macrophages; No productions; Ultrasonic treatments; Polysaccharides

Abstract :

[en] Ultrasonic treatment was performed on water-extractable polysaccharides from the seed of mung beans. Purified by anion-exchange and gel filtration chromatography, MWP-1′ and MWP-2′ were obtained. Average molecular weights (Mws) of MWP-1′ and MWP-2′ were 68.4 kDa, and 52.4 kDa, respectively. Monosaccharides components analysis indicated that MWP-1′ was composed of Rha, Ara, Man and Gal in a molar percent of 0.4:2.6:5.3:0.7. MWP-2′ was composed of Ara, Man, Gal and Glc in a molar percent of 0.5:1.4:2.1:0.4. In vitro study showed that both polysaccharides samples were able to stimulate the production of secretory molecules (NO, TNF-α and IL-6) of RAW264.7 murine macrophages in a dosage dependent manner. MWP-2′ seemed to be the most potent and induced significantly higher the NO production. These findings suggest that the ultrasonic treatment polysaccharides isolated in our study have immune potentiation effects on macrophages. © 2015 Elsevier Ltd. All rights reserved.

Disciplines :

Agriculture & agronomy

Author, co-author :

Yao, Y.; Institute of Crop Science, Chinese Academy of Agricultural Sciences, No. 80 South Xueyuan Road, Haidian District, Beijing, China

Zhu, Yingying ; Université de Liège - ULiège > Doct. sc. agro. & ingé. biol. (Paysage)

Ren, G.; Institute of Crop Science, Chinese Academy of Agricultural Sciences, No. 80 South Xueyuan Road, Haidian District, Beijing, China

Language :

English

Title :

Immunoregulatory activities of polysaccharides from mung bean

Publication date :

2016

Journal title :

Carbohydrate Polymers

ISSN :

0144-8617

eISSN :

1879-1344

Publisher :

Elsevier, United Kingdom

Volume :

139

Pages :

61-66

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

Hong Kong, Macao andTaiwan Science & Technology Cooperation Program of China (2014DFT30280, 2013DFH30050); Special fund for Agro-scientific Research in the Public Interest (201403063); Earmarked fund for China Agriculture Research System Modern Agro-industry Technology (CYTX-014); Agricultural Science and Technology Program for Innovation Team on Identification and Excavation of Elite Crop Germplasm, CAAS.

Funders :

CAAS - Chinese Academy of Agricultural Sciences

Available on ORBi :

since 07 November 2020

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