Article (Scientific journals)
Detection of lunasin in quinoa (Chenopodium quinoa Willd.) and the in vitro evaluation of its antioxidant and anti-inflammatory activities
Ren, G.; Zhu, Yingying; Shi, Zhenxing et al.
2017In Journal of the Science of Food and Agriculture, 97 (12), p. 4110-4116
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Keywords :
Chenopodium quinoa; RAW 264.7 cell line; Animals; Anti-Inflammatory Agents; Antioxidants; Chromatography, High Pressure Liquid; Interleukin-6; Macrophages; Mass Spectrometry; Mice; Nitric Oxide; Plant Extracts; RAW 264.7 Cells; Seeds; Tumor Necrosis Factor-alpha
Abstract :
[en] BACKGROUND: Lunasin is a novel cancer-preventive peptide that has been detected in various plants. However, the presence and bioactivity of lunasin in quinoa have not been demonstrated. RESULTS: Lunasin was detected in quinoa using ultrahigh-pressure liquid chromatography–electrosparay ionization–mass spectrometry. The content in 15 quinoa samples ranged from 1.01 × 10−3 g kg−1 dry seed to 4.89 × 10−3 g kg−1 dry seed. Significant differences (P < 0.05) in lunasin content among different cultivars (yy22, xsg86, xsg59, yy30 and yy19) from the same area and the same cultivar from different areas (Hainan, Hebei and Shanxi provinces) were observed. The lunasin isolated and purified from quinoa (QLP) exhibited a weak DPPH radical scavenging activity (no IC50 value), but a strong ABTS+ radical scavenging activity (IC50 value, 1.45 g L−1) and oxygen radical scavenging activity (40.06 µmol L−1 Trolox equivalents/g QLP when the concentration was 3.20 g L−1). In addition, QLP inhibited the production of nitric oxide (NO), tumor necrosis factor-α and interleukin-6 on lipopolysaccharide-stimulated RAW264.7 macrophages by up to 44.77%, 39.81% and 33.50%, respectively, at a concentration of 0.40 g L−1. CONCLUSION: Taken together, these findings indicate that lunasin presents in quinoa and is bioactive, which strengthens the recommendations for the development of quinoa-based functional foods. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry
Disciplines :
Agriculture & agronomy
Author, co-author :
Ren, G.;  College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, China, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China
Zhu, Yingying ;  Université de Liège - ULiège > Doct. sc. agro. & ingé. biol. (Paysage)
Shi, Zhenxing ;  Université de Liège - ULiège > Terra
Li, Jianhui
Language :
English
Title :
Detection of lunasin in quinoa (Chenopodium quinoa Willd.) and the in vitro evaluation of its antioxidant and anti-inflammatory activities
Publication date :
2017
Journal title :
Journal of the Science of Food and Agriculture
ISSN :
0022-5142
eISSN :
1097-0010
Publisher :
John Wiley and Sons Ltd
Volume :
97
Issue :
12
Pages :
4110-4116
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
International Science and Technology Cooperation Program of China (KY20142023); Hong Kong, Macao, and Taiwan Science and Technology Cooperation Program of China (2013DFH30050); Science and Technology Planning Project of General Administration of Quality Supervision, Inspection and Quarantine (AQSIQ) of P.R. China (2015IK012, 2016IK021)
Funders :
ULiège. GxABT - Liège Université. Gembloux Agro-Bio Tech [BE]
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