Evaluation of the new Id-Fungi plates from Conidia for MALDI-TOF MS identification of filamentous fungi and comparison with conventional methods as identification tool for dermatophytes from nails, hair and skin samples

SACHELI, Rosalie; Henri, Anne-Sophie; SEIDEL, Laurence; ERNST, Marie; DARFOUF, Rajae; ADJETEY BAHUN, Akolé; SCHYNS, Marjorie; MARECHAL, Ludwig; MEEX, Cécile; ARRESE ESTRADA, Jorge; HAYETTE, Marie-Pierre

doi:10.1111/myc.13156

Article (Scientific journals)

Evaluation of the new Id-Fungi plates from Conidia for MALDI-TOF MS identification of filamentous fungi and comparison with conventional methods as identification tool for dermatophytes from nails, hair and skin samples

SACHELI, Rosalie; Henri, Anne-Sophie; SEIDEL, Laurence et al.

2020 • In Mycoses

Peer Reviewed verified by ORBi

Permalink
https://hdl.handle.net/2268/252104

DOI
10.1111/myc.13156

PubMed
32757444

Files (1)Send to Details Statistics Bibliography Similar publications

Files

Full Text

Papier publié Sacheli.pdf

Publisher postprint (979.88 kB)

Download

All documents in ORBi are protected by a user license.

Send to

RIS BibTex APA Chicago Permalink X Linkedin

Details

Keywords :

dermatophytes; Id-Fungi plates; MALDI-TOF MS

Abstract :

[en] Objectives: We first compare the efficiency of mould/dermatophytes identification by MALDI-TOF MS using a new medium called Id-Fungi plates (IDFP) from Conidia® and two different databases. For the second purpose, we evaluated a new version of the medium supplemented with cycloheximide, Id-Fungi plates Plus (IDFPC) for the direct inoculation of nails, hair and skin samples and compared the efficiency of MALDI-TOF MS identification of dermatophytes to classical methods based on culture and microscopy. Methods: A total of 71 strains have been cultured IDFP and Sabouraud gentamicin plates (SGC2) and were identified by MALDI-TOF MS. For the evaluation of the combination IDFPC/ MALDI-TOF MS as a method of identification for dermatophytes, 428 samples of hair nails and skin were cultivated in parallel on IDFPC and Sabouraud + cycloheximide medium (SAB-ACTI ). Results: For Aspergillus sp. and non-Aspergillus moulds, the best performances were obtained on IDFP after maximum 48h growth, following protein extraction. For dermatophytes, the best condition was using the IDFP at 72h, after extended direct deposit. Regarding the direct inoculation of nails, hair skin on IDFPC, 129/428 (30.1%) showed a positive culture against 150/428 (35%) on SAB-ACTI medium. Among the 129 positive strains the identification by MALDI-TOF MS was correct for 92/129 (71.4%). Conclusion: The IDFP allows the generation of better spectra by MALDI-TOF MS compared to SGC2. It facilitates sampling and deposit. Regarding the use of IDFPC, this medium seems less sensitive than SAB-ACTI but among positive strains, the rate of correct identification, by MALDI-TOF MS is satisfactory.

Disciplines :

Microbiology

Author, co-author :

SACHELI, Rosalie ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire CNR

Henri, Anne-Sophie

SEIDEL, Laurence ; Centre Hospitalier Universitaire de Liège - CHU > Département de gestion des systèmes d'informations (GSI) > Secteur d'appui à la recherche clinique et biostatistique

ERNST, Marie ; Centre Hospitalier Universitaire de Liège - CHU > Département de gestion des systèmes d'informations (GSI) > Secteur d'appui à la recherche clinique et biostatistique

DARFOUF, Rajae ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire CNR

ADJETEY BAHUN, Akolé ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Unité de laboratoire - LRS Biomol

SCHYNS, Marjorie ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire dispatching et mise en culture

MARECHAL, Ludwig ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Discipline Anatomie et Cytologie Pathologiques (Anatomie Pathologique et Dermatologie)

MEEX, Cécile ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire bactériologie

ARRESE ESTRADA, Jorge ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Service dermatopathologie

HAYETTE, Marie-Pierre ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire parasitologie

Language :

English

Title :

Publication date :

July 2020

Journal title :

Mycoses

ISSN :

0933-7407

eISSN :

1439-0507

Publisher :

Blackwell, Oxford, United Kingdom

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 30 October 2020

Statistics

Number of views

169 (24 by ULiège)

Number of downloads

423 (13 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

Bibliography

Carbonnelle E, Mesquita C, Bille E, et al. MALDI-TOF mass spectrometry tools for bacterial identification in clinical microbiology laboratory. Clin Biochem. 2011;44:104-109.
Bizzini A, Greub G. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification. Clin Microbiol Infect. 2010;16:1614-1619.
Clark AE, Kaleta EJ, Arora A, Wolk DM. Matrix-Assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology. Clin Microbiol Rev. 2013;26:547-603.
Dhiman N, Hall L, Wohlfiel SL, Buckwalter SP, Wengenack NL. Performance and cost analysis of matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine identification of yeast. J Clin Microbiol. 2011;49:1614-1616.
Van Veen SQ, Claas ECJ, Kuijper EJ. High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories. J Clin Microbiol. 2010;48:900-907.
Hedayati MT, Ansari S, Ahmadi B, et al. Identification of clinical dermatophyte isolates obtained from Iran by matrix-assisted laser desorption/ionization time-of light mass spectrometry. Curr Med Mycol. 2019;5(2):22-26.
L’Ollivier C, Ranque S. MALDI-TOF-Based Dermatophyte Identification. Mycopathologia. 2017;182(1–2):183-192.
Nenoff P, Erhard M, Simon JC, et al. MALDI-TOF mass spectrometry - a rapid method for the identification of dermatophyte species. Med Mycol. 2013;51:17-24.
De Respinis S, Monnin V, Girard V, et al. Matrix-assisted laser desorption ionization-time of flight (MALDITOF) mass spectrometry using the Vitek MS system for rapid and accurate identification of dermatophytes on solid cultures. J Clin Microbiol. 2014;52:4286-4292.
Lau AF, Drake SK, Calhoun LB, Henderson CM, Zelazny AM. Development of a clinically comprehensive database and a simple procedure for identification of molds from solid media by matrix-assisted laser desorption ionization-Time of flight mass spectrometry. J Clin Microbiol. 2013;51:828-834.
Lachaud L, Fernández-Arévalo A, Normand AC, et al. Identification of Leishmania by MALDI-TOF mass spectrometry using a free web-based application and a dedicated mass spectral library. J Clin Microbiol. 2017;55(10):2924–2933.
Ferrer C, Colom F, Frasés S, Mulet E, Abad JL, Alió JL. Detection and identification of fungal pathogens by PCR and by ITS2 and 5.8S ribosomal DNA typing in ocular infections. J Clin Microbiol. 2001;39:2873-2879.
Normand A-C, Cassagne C, Gautier M, et al. Decision criteria for MALDI-TOF MS-based identification of filamentous fungi using commercial and in-house reference databases. BMC Microbiol. 2017;17(1):25.
Normand AC, Becker P, Gabriel F, et al. Validation of a new web application for identification of fungi by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2017;55:2661-2670.
Vidal-Acuña MR, Ruiz-Pérez De Pipaón M, Torres-Sánchez MJ, Aznar J. Identification of clinical isolates of Aspergillus, including cryptic species, by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Med Mycol. 2018;56:838-846.
De Carolis E, Posteraro B, Lass-Flörl C, et al. Species identification of Aspergillus, Fusarium and Mucorales with direct surface analysis by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Microbiol Infect. 2012;18:475-484.
Park JH, Shin JH, Choi MJ, et al. Evaluation of matrix-assisted laser desorption/ionization time-of-fight mass spectrometry for identification of 345 clinical isolates of Aspergillus species from 11 Korean hospitals: comparison with molecular identification. Diagn Microbiol Infect Dis. 2017;87:28-31.
McMullen A, Wallace M, Pincus D, Wilkey K, Burnham C-A. Evaluation of the VITEK MS MALDI-TOF MS system for identification of clinically relevant filamentous fungi. J Clin Microbiol. 2016;54(8):2068–2073.
Rychert J, Slechta ES, Barker AP, et al. Multicenter evaluation of the Vitek MS v3.0 system for the identification of filamentous fungi. J Clin Microbiol. 2018;56(2):e01353-17
Packeu A, De Bel A, l’Ollivier C, Ranque S, Detandt M, Hendrickx M. Fast and accurate identification of dermatophytes by MALDI-TOF mass spectrometry: validation in the clinical lab. J Clin Microbiol. 2014;52(12):4286–4292.
Intra J, Sarto C, Tiberti N, Besana S, Savarino C, Brambilla P. Genus-level identification of dermatophytes by MALDI-TOF MS after 2 days of colony growth. Lett Appl Microbiol. 2018;67:136-143.
Schulthess B, Ledermann R, Mouttet F, et al. Use of the Bruker MALDI Biotyper for identification of molds in the clinical mycology laboratory. J Clin Microbiol. 2014;52:2797-2803.
Reich M. Species identification of bacteria and fungi from solid and liquid culture media by MALDI-TOF mass spectrometry. J Bacteriol Parasitol. 2013;01:S5-002.
Masih A, Singh PK, Kathuria S, Agarwal K, Meis JF, Chowdhary A. Identification by molecular methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry and antifungal susceptibility profiles of clinically significant rare aspergillus species in a referral chest hospital in Delhi, India. J Clin Microbiol. 2016;54:2354-2364.
Stein M, Tran V, Nichol KA, et al. Evaluation of three MALDI-TOF mass spectrometry libraries for the identification of filamentous fungi in three clinical microbiology laboratories in Manitoba, Canada. Mycoses. 2018;61:743-753.
Patel R. A moldy application of MALDI: MALDI-ToF mass spectrometry for fungal identification. J Fungi. 2019;5:4.
L’Ollivier C, Cassagne C, Normand A-C, et al. A MALDI-TOF MS procedure for clinical dermatophyte species identification in the routine laboratory. Med Mycol. 2013;51:713-720.
Zvezdanova ME, Escribano P, Ruiz A, et al. Increased species-assignment of filamentous fungi using MALDI-TOF MS coupled with a simplified sample processing and an in-house library. Med Mycol. 2019;57:63-70.
da Cunha KC, Riat A, Normand A-C, et al. Fast identification of dermatophytes by MALDI-TOF/MS using direct transfer of fungal cells on ground steel target plates. Mycoses. 2018;61:691-697.
Calderaro A, Motta F, Montecchini S, et al. Identification of Dermatophyte Species after implementation of the in-house MALDI-TOF MS database. Int J Mol Sci. 2014;15:16012-16024.
Karabıçak N, Karatuna O, İlkit M, Akyar I. Evaluation of the bruker matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) system for the identification of clinically important dermatophyte species. Mycopathologia. 2015;180:165-171.
Sleiman S, Halliday CL, Chapman B, et al. Performance of matrix-assisted laser desorption ionization time of flight mass spectrometry for identification of aspergillus, scedosporium, and fusarium spp. in the australian clinical setting. J Clin Microbiol. 2016;54:2182-2186.
Becker P, Normand A-C, Vanantwerpen G, et al. Identification of fungal isolates by MALDI-TOF mass spectrometry in veterinary practice: validation of a web application. J Vet Diagnostic Investig. 2019;31:471-474.
De Respinis S, Tonolla M, Pranghofer S, Petrini L, Petrini O, Bosshard PP. Identification of dermatophytes by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Med Mycol. 2013;51:514-521.
De Respinis S, Monnin V, Girard V, et al. MALDI-TOF VITEK MS for the rapid and accurate identification of dermatophytes on solid cultures. J Clin Microbiol. 2014;52(12):4286–4292.
Lau AF, Walchak RC, Miller HB, et al. Multicenter study demonstrates standardization requirements for mold identification by MALDI-TOF MS. Front Microbiol. 2019;10:2098.
Cassagne C, Normand AC, L’Ollivier C, Ranque S, Piarroux R. Performance of MALDI-TOF MS platforms for fungal identification. Mycoses. 2016;59:678-690.
Vanbeckhoven D., Sacheli R., Patteet S., Hayette MP, Lagrou K. 2016-2017 report of the Belgian National Reference Center for Mycoses. 2019 https://doi.org/10.25608/0eb9-2439
Sacheli R, Adjetey C, Darfouf R, et al. A one-year survey of Microsporum audouinii infections in Belgium: epidemiological and genotypic characterization. Clin Microbiol Infect. 2016;22:285.e9-285.e17.
Brito-Santos F, Figueiredo-Carvalho MHG, Coelho RA, Sales A, Almeida-Paes R. Tinea Capitis by Microsporum audouinii: case reports and review of published global literature 2000–2016. Mycopathologia. 2017;182:1053-1060.
Ginter-Hanselmayer G, Weger W, Ilkit M, Smolle J. Epidemiology of tinea capitis in Europe: current state and changing patterns. Mycoses. 2007;50:6-13.
Panasiti V, Devirgiliis V, Borroni RG, et al. Epidemiology of dermatophytic infections in Rome, Italy: a retrospective study from 2002 to 2004. Med Mycol. 2002;45:57-60.
Rezusta A, Betrán A, Querol I, Palacián MP, Revillo MJ. Tinea capitis caused by Trichophyton soudanense and Microsporum audouinii in an adult: a case report. Mycoses. 2011;54:89-90.
de Hoog GS, Dukik K, Monod M, et al. Toward a novel multilocus phylogenetic taxonomy for the Dermatophytes. Mycopathologia. 2017;182:5-31.
Yamada T, Maeda M, Alshahni MM, et al. Terbinafine resistance of Trichophyton clinical isolates caused by specific point mutations in the squalene epoxidase gene. Antimicrob Agents Chemother. 2017;61(7):e00115-17.
Singh A, Masih A, Khurana A, et al. High terbinafine resistance in Trichophyton interdigitale isolates in Delhi, India harbouring mutations in the squalene epoxidase gene. Mycoses. 2018;61(7):477–484.
Cassagne C, Ranque S, Normand A-C, et al. Mould routine identification in the clinical laboratory by Matrix-Assisted laser desorption ionization Time-Of-Flight mass spectrometry. PLoS One. 2011;6(12):e28425.
Posteraro B, De Carolis E, Vella A, Sanguinetti M. MALDI-TOF mass spectrometry in the clinical mycology laboratory: Identification of fungi and beyond. Expert Rev Proteomics. 2013;10(2):151-164.
Bader O. MALDI-TOF-MS-based species identification and typing approaches in medical mycology. Proteomics. 2013;13:788-799.