Methyl-beta-cyclodextrin concurs with interleukin (IL)-4, IL-13 and IL-25 to induce alterations reminiscent of atopic dermatitis in reconstructed human epidermis.
De Vuyst, Evelyne; Giltaire, Severine; Lambert de Rouvroit, Catherineet al.
2018 • In Experimental Dermatology, 27 (4), p. 435-437
Methyl-beta-cyclodextrin concurs with interleukin (IL)-4, IL-13 and IL-25 to induce alterations reminiscent of atopic dermatitis in reconstructed human epidermis..pdf
atopic dermatitis; epidermal barrier and methyl-beta-cyclodextrin; interleukins; reconstructed human epidermis
Abstract :
[en] Reconstructed human epidermis (RHE) mimic normal human in vivo epidermis in terms of histology, distribution of differentiation markers and barrier functionality. A typical transcriptional profile and the activation of signalling pathways reminiscent of atopic dermatitis (AD) lesional skin can be obtained in RHE upon incubation with methyl-β-cyclodextrin (MβCD), a molecule that extracts cholesterol from plasma membranes, thereby disrupting lipid microdomains. However, barrier function and morphology remain unaltered in those conditions, requiring further refinement of the model.
Disciplines :
Dermatology
Author, co-author :
De Vuyst, Evelyne
Giltaire, Severine
Lambert de Rouvroit, Catherine
Malaisse, Jeremy
Mound, Abdallah
Bourtembourg, Maureen
Poumay, Yves
NIKKELS, Arjen ; Centre Hospitalier Universitaire de Liège - CHU > Autres Services Médicaux > Service de dermatologie
Chretien, Aline
Salmon, Michel
Language :
English
Title :
Methyl-beta-cyclodextrin concurs with interleukin (IL)-4, IL-13 and IL-25 to induce alterations reminiscent of atopic dermatitis in reconstructed human epidermis.