Article (Scientific journals)
Novel dipeptidyl peptidase-IV and angiotensin-I-converting enzyme inhibitory peptides released from quinoa protein by in silico proteolysis
Guo, Huimin; Richel, Aurore; Hao, Y. et al.
2020In Food Science and Nutrition, 8 (3), p. 1415-1422
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Keywords :
ACE inhibitors; DPP-IV inhibitors
Abstract :
[en] Quinoa protein has been paid more and more attention because of its nutritional properties and beneficial effects. With the development of bioinformatics, bioactive peptide database and computer-assisted simulation provide an efficient and time-saving method for the theoretical estimation of potential bioactivities of protein. Therefore, the potential of quinoa protein sequences for releasing bioactive peptides was evaluated using the BIOPEP database, which revealed that quinoa protein, especially globulin, is a potential source of peptides with dipeptidyl peptidase-IV (DPP-IV) and angiotensin-I-converting enzyme (ACE) inhibitory activities. Three plant proteases, namely papain, ficin, and stem bromelain, were employed for the in silico proteolysis of quinoa protein. Furthermore, four tripeptides (MAF, NMF, HPF, and MCG) were screened as novel promising bioactive peptides by PeptideRanker. The bioactivities of selected peptides were confirmed using chemical synthesis and in vitro assay. The present work suggests that quinoa protein can serve as a good source of bioactive peptides, and in silico approach can provide theoretical assistance for investigation and production of functional peptides. © 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc.
Disciplines :
Agriculture & agronomy
Author, co-author :
Guo, Huimin ;  Université de Liège - ULiège > Terra
Richel, Aurore  ;  Université de Liège - ULiège > Département GxABT > SMARTECH
Hao, Y.;  Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China
Fan, X.;  Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium
Everaert, Nadia ;  Université de Liège - ULiège > Département GxABT > Ingénierie des productions animales et nutrition
Yang, X.;  Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China
Ren, G.;  Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China
Language :
English
Title :
Novel dipeptidyl peptidase-IV and angiotensin-I-converting enzyme inhibitory peptides released from quinoa protein by in silico proteolysis
Publication date :
2020
Journal title :
Food Science and Nutrition
eISSN :
2048-7177
Publisher :
Wiley-Blackwell
Volume :
8
Issue :
3
Pages :
1415-1422
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Chinese Academy of Agricultural Sciences, CAAS19227527D-01International Science and Technology Cooperation Programme, ISTCP: KY20142023
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since 22 September 2020

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