Reference : State of art and best practices for fatty acid analysis in aquatic sciences
Scientific journals : Article
Life sciences : Environmental sciences & ecology
Life sciences : Aquatic sciences & oceanology
Life sciences : Zoology
State of art and best practices for fatty acid analysis in aquatic sciences
Couturier, Lydie I. E.* []
Michel, Loïc* mailto [Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Département de Biologie, Ecologie et Evolution >]
Amaro, Teresa []
Budge, Suzanne M. []
da Costa, Elizabete []
De Troch, Marleen []
Di Dato, Valeria []
Fink, Patrick []
Giraldo, Carolina []
Le Grand, Fabienne []
Loaiza, Ivan []
Mathieu-Resuge, Margaux []
Nichols, Peter D. []
Parrish, Christopher C. []
Sardenne, Fany []
Vagner, Marie []
Pernet, Fabrice* []
Soudant, Philippe* []
* These authors have contributed equally to this work.
ICES Journal of Marine Science
Oxford University Press
Yes (verified by ORBi)
United Kingdom
[en] aquatic nutrition ; biomarkers ; fatty acids ; marine and freshwater lipids ; methods ; recommended practices
[en] Determining the lipid content and fatty acid (FA) composition of aquatic organisms has been of major interest in trophic ecology, aquaculture, and nutrition for over half a century. Although protocols for lipid analysis are well-described, their application to aquatic sciences often requires modifications to adapt to field conditions and to sample type. Here, we present the current state of knowledge of methods dedicated to both marine and freshwater lipid analyses, from sampling to data treatment. We review: (i) sample preservation, storage and transport protocols, and their effects on lipids, (ii) lipid extraction, separation of polar and neutral lipids, derivatization, and detection methods, and (iii) available tools for the statistical analysis of FA data. We provide recommendations for best practices in field situations and advocate for protocol standardization and interlaboratory calibration.
Freshwater and OCeanic science Unit of reSearch - FOCUS ; Centre Interfacultaire de Recherches en Océanologie - MARE
Researchers ; Professionals ; Students

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