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18 September 2020
Article (Scientific journals)
State of art and best practices for fatty acid analysis in aquatic sciences
Couturier, Lydie I. E.; Michel, Loïc; Amaro, Teresa et al.
2020In ICES Journal of Marine Science, 77 (7-8), p. 2375-2395
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Keywords :
aquatic nutrition; biomarkers; fatty acids; marine and freshwater lipids; methods; recommended practices
Abstract :
[en] Determining the lipid content and fatty acid (FA) composition of aquatic organisms has been of major interest in trophic ecology, aquaculture, and nutrition for over half a century. Although protocols for lipid analysis are well-described, their application to aquatic sciences often requires modifications to adapt to field conditions and to sample type. Here, we present the current state of knowledge of methods dedicated to both marine and freshwater lipid analyses, from sampling to data treatment. We review: (i) sample preservation, storage and transport protocols, and their effects on lipids, (ii) lipid extraction, separation of polar and neutral lipids, derivatization, and detection methods, and (iii) available tools for the statistical analysis of FA data. We provide recommendations for best practices in field situations and advocate for protocol standardization and interlaboratory calibration.
Research center :
FOCUS - Freshwater and OCeanic science Unit of reSearch - ULiège
MARE - Centre Interfacultaire de Recherches en Océanologie - ULiège
Disciplines :
Aquatic sciences & oceanology
Environmental sciences & ecology
Author, co-author :
Couturier, Lydie I. E. 
Michel, Loïc   ;  Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Département de Biologie, Ecologie et Evolution
Amaro, Teresa
Budge, Suzanne M.
da Costa, Elizabete
De Troch, Marleen
Di Dato, Valeria
Fink, Patrick
Giraldo, Carolina
Le Grand, Fabienne
Loaiza, Ivan
Mathieu-Resuge, Margaux
Nichols, Peter D.
Parrish, Christopher C.
Sardenne, Fany
Vagner, Marie
Pernet, Fabrice 
Soudant, Philippe 
More authors (8 more) Less
 These authors have contributed equally to this work.
Language :
Title :
State of art and best practices for fatty acid analysis in aquatic sciences
Publication date :
December 2020
Journal title :
ICES Journal of Marine Science
Publisher :
Oxford University Press, United Kingdom
Volume :
Issue :
Pages :
Peer reviewed :
Peer Reviewed verified by ORBi


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