Screening of pesticide residues in Traditional Chinese Medicines using modified QuEChERS sample preparation procedure and LC-MS/MS analysis

Li, R.-X.; Li, M.-M.; Wang, T.; Wang, T.-L.; Chen, J.-Y.; Francis, Frédéric; Fan, B.; Kong, Z.-Q.; Dai, X.-F.

doi:10.1016/j.jchromb.2020.122224

Article (Scientific journals)

Screening of pesticide residues in Traditional Chinese Medicines using modified QuEChERS sample preparation procedure and LC-MS/MS analysis

Li, R.-X.; Li, M.-M.; Wang, T. et al.

2020 • In Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 1152

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https://hdl.handle.net/2268/250777

DOI
10.1016/j.jchromb.2020.122224

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32559653

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Keywords :

Modified QuEChERS; Multiresidues; Pesticides; Traditional Chinese Medicine; UHPLC-MS/MS; Extraction; Magnesium compounds; Medicine; Phase separation; Screening; Dispersive solid phase extraction; High-throughput method; Limits of quantitations; Maximum residue levels; Ophiopogon Japonicus; Pesticide residue; Sample preparation procedure

Abstract :

[en] A robust and high-throughput method was developed for the determination of 108 pesticide residues in Traditional Chinese Medicines (TCMs) simultaneously using a combination of UHPLC-MS/MS analysis and the modified QuEChERS method. Extraction was carried out in acetonitrile containing 0.75% (v/v) acetic acid with ultrasonication for 15 min; MgSO4 and C18 were used as the dispersive-solid phase extraction sorbents. The method exhibited good linearity (r2 > 0.9901), in addition to good selectivity, precision and repeatability. More than 92% of pesticides exhibited high rates or recovery in the 70–120% range. This method showed high sensitivity, with Limits of Quantitation in the 0.01–20 ng/mL range in Cortex Moutan, and 0.01–50 ng/mL in the other TCMs. The method was employed for the analysis of 39 real samples from different habitats, and pesticides were detected in 92.3% of the samples, with 26 pesticides being detected in these three TCMs. More than four pesticides were detected in a third of the samples. Among them, tebuconazole was detected in all the three TCMs with 0.22–22.02 μg/kg concentration, which was lower than the provisions in GB 2763-2019 (50 μg/kg). In addition, the paclobutrazol detection rate in Ophiopogon japonicus was 100%, and the detected concentrations of 9 samples exceeded the Maximum Residue Levels defined for vegetables (50 μg/kg). Considering there are no regulations that govern the limits of pesticide residues in the three TCMs in China, we recommend the acceleration of efforts to introduce appropriate regulations. © 2020 Elsevier B.V.

Disciplines :

Agriculture & agronomy

Author, co-author :

Li, R.-X.; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Li, M.-M.; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Wang, T.; Institute of Chinese Medicinal Materials, Mianyang Academy of Agricultural Sciences, Mianyang, 621023, China

Wang, T.-L.; National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, State Key Laboratory Breeding Base of Dao-di Herbs, Beijng, 100700, China

Chen, J.-Y.; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Francis, Frédéric ; Université de Liège - ULiège > Département GxABT > Gestion durable des bio-agresseurs

Fan, B.; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Kong, Z.-Q.; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Dai, X.-F.; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Language :

English

Title :

Screening of pesticide residues in Traditional Chinese Medicines using modified QuEChERS sample preparation procedure and LC-MS/MS analysis

Publication date :

2020

Journal title :

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

ISSN :

1570-0232

eISSN :

1873-376X

Publisher :

Elsevier B.V.

Volume :

1152

Peer reviewed :

Peer Reviewed verified by ORBi

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since 09 September 2020

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