Development of new dermatological formulations for the treatment of cutaneous candidiasis

[en] The inhibitory activity of the essential oils (EO) of mint and thyme on Candida albicans are well known, however, their valorization in a dosage form for the management of cutaneous candidiasis has been little explored. This study aimed to formulate innovative and cost-effective dermatological topicals based on mint and thyme essential oils for the treatment of cutaneous candidiasis. Thyme essential oil was obtained commercially, while that of mint was obtained by hydrodistillation. In order to determine their antifungal activity, both essentials oils were tested alone and in combination against a reference strain of Candida albicans (strain MHMR) and clinical strains of Candida albicans and Candida tropicalis. Three creams were formulated using natural (shea butter, palm oil) and chemical (cetomacrogol) excipients. The efficacies of these creams were assessed in vivo using Wistar rats infected with Candida albicans MHMR. The in vitro antimicrobial study showed that C. albicans MHMR was more sensitive to thyme essential oil with a MIC of 310 μgmL−1, which was higher than the reference fluconazole used for the in vitro study (32 μgmL−1). The antimicrobial synergy study of both essential oils using checkerboard test demonstrated an additive effect of mint and thyme essential oils on C. albicans MHMR. GC/FID and GC/MS analyses led to the identification of thymol and menthol respectively as the main components of thyme and mint essential oils. A shea butter cream base and a shea butter and palm oil cream both containing 5% of EO (mint and thyme) exhibited the best in vivo antimicrobial activities, inducing optimal wound healing in infected rats compared with ketoconazole commercial cream used for the in vivo study. These results provide a solid basis at least in part, for the use of essential oil in creams formulated with natural excipients for the management of cutaneous candidiasis.

Disciplines :

Pharmacy, pharmacology & toxicology

Author, co-author :

Zinsou, Aude

Assanhou, Gabin

Ganfon, Habib

Sounouvou, Axel Gérald Hope Tognidé ; Université de Liège - ULiège > Doct. sc. bioméd. & pharma. (paysage)

Kassehin, Urbain

Lawson, Roland

Medegan, Sedami

Evrard, Brigitte ; Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique

Quetin-Leclercq, Joëlle

Language :

English

Title :

Development of new dermatological formulations for the treatment of cutaneous candidiasis

Publication date :

2020

Journal title :

Scientific African

eISSN :

2468-2276

Publisher :

Elsevier, Netherlands

Volume :

Pages :

e00342 [1-8]

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 31 August 2020

Statistics

Number of views

72 (6 by ULiège)

Number of downloads

4 (4 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

Bibliography

van, Hees C, B, Naafs, Common skin diseases in Africa: an illustrated guide. Voorburg, Lelystad, 2001.
WHO, Epidemiology and management of common skin diseases in children in develping country. WHO/FCH/CAH/05.12. www.who.int/maternal_child_adolescent/documents/fch_cah_05_12/en/.
del Palacio, A., Cuétara, S., Pérez, A., Garau, M., Calvo, T., Sánchez-Alor, G, Topical treatment of dermatophytosis and cutaneous candidosis with flutrimazole 1% cream: double-blind, randomized comparative trial with ketoconazole 2% cream. Mycoses 42 (1999), 649–655.
Dieng, Y., Faye-Niang, M.A., Ndour-Diop, A., Sow, P.S., Dieng, T., Soumare, M., et al. [Antifungal drug susceptibility of Candida causing oropharyngeal candidiasis in HIV infected patients]. Dakar Med. 46 (2001), 4–7.
Randhawa, M.A., Alghamdi, M.S., Anticancer activity of nigella sativa (Black seed)–a review. Am. J. Chin. Med. (Gard City NY) 39 (2011), 1075–1091, 10.1142/S0192415X1100941X.
Orchard, A., van Vuuren, S, Commercial essential oils as potential antimicrobials to treat skin diseases. Evid.-Based Complementary Alternative Med. 2017 (2017), 1–92, 10.1155/2017/4517971.
Giordani, R., Regli, P., Kaloustian, J., Mikaïl, C., Abou, L., Portugal, H, Antifungal effect of various essential oils against candida albicans. Potentiation of antifungal action of amphotericin B by essential oil from thymus vulgaris: antifungal effect of various of essential oils. Phytotherapy Res. 18 (2004), 990–995, 10.1002/ptr.1594.
Rahimifard, N., Sabzevari, O., Shoeibi, S., Pakzad, S., Ajdary, S., Haghighi, S., et al. Antifungal activity of the native essential oil of Zataria multiflora on Candida albicans. Aspergillus Niger and Aspergillus Flavus from Iran, 1, 2008, 4.
das Neves, J., Pinto, E., Amaral, M.H., Bahia, M.F, Antifungal activity of a gel containing thymus vulgaris essential oil against Candida species commonly involved in vulvovaginal candidosis. Pharm. Biol. 47 (2009), 151–153, 10.1080/13880200802436232.
Işcan, G., Kirimer, N., Kürkcüoğlu, M., Başer, K.H.C., Demirci, F, Antimicrobial screening of Mentha piperita essential oils. J. Agric. Food Chem. 50 (2002), 3943–3946.
Gutierrez, J., Barry-Ryan, C., Bourke, P, The antimicrobial efficacy of plant essential oil combinations and interactions with food ingredients. Int. J. Food Microbiol. 124 (2008), 91–97, 10.1016/j.ijfoodmicro.2008.02.028.
Turgis, M., Vu, K.D., Dupont, C., Lacroix, M, Combined antimicrobial effect of essential oils and bacteriocins against foodborne pathogens and food spoilage bacteria. Food Res. Int. 48 (2012), 696–702, 10.1016/j.foodres.2012.06.016.
Mulyaningsih, S., Sporer, F., Zimmermann, S., Reichling, J., Wink, M, Synergistic properties of the terpenoids aromadendrene and 1,8-cineole from the essential oil of Eucalyptus globulus against antibiotic-susceptible and antibiotic-resistant pathogens. Phytomedicine 17 (2010), 1061–1066, 10.1016/j.phymed.2010.06.018.
Ray, T.L., Wuepper, K.D., Experimental cutaneous candidiasis in rodents; II. Role of the stratum corneum barrier and serum complement as a mediator of a protective infalmmatory response. Arch. Dermatol. 114 (1978), 539–543.
Aligiannis, N., Kalpoutzakis, E., Chinou, I.B., Mitakou, S., Gikas, E., Tsarbopoulos, A, Composition and antimicrobial activity of the essential oils of five taxa of sideritis from Greece. J. Agric. Food Chem. 49 (2001), 811–815, 10.1021/jf001018w.
Duarte, M.C.T., Figueira, G.M., Sartoratto, A., Rehder, V.L.G., Delarmelina, C, Anti-Candida activity of Brazilian medicinal plants. J. Ethnopharmacol. 97 (2005), 305–311, 10.1016/j.jep.2004.11.016.
Mahboubi, M., Kazempour, N., Chemical composition and antimicrobial activity of peppermint (Mentha piperita L.) Essential oil. Songklanakarin J. Sci. Technol. 36:1 (2014), 83–87.
Walker, H.L., Mason, A.D, A standard animal burn. J. Trauma. 8 (1968), 1049–1051.
Sasidharan, S., Nilawatyi, R., Xavier, R., Latha, L.Y., Amala, R, Wound healing potential of elaeis guineensis JACQ leaves in an infected albino rat model. Molecules 15 (2010), 3186–3199, 10.3390/molecules15053186.
Barku, V.Y.A., Boye, A., Ayaba, S, Phytochemical screening and assessment of wound healing activity of the leaves of Anogeissus leiocarpus. Eur. J. Exp. Biol. 3 (2013), 18–25.
Giandoni, M.B., Grabski, W.J., Cutaneous candidiasis as a cause of delayed surgical wound healing. J. Am. Acad. Dermatol. 30 (1994), 981–984, 10.1016/S0190-9622(94)70122-9.
Vermeulen, H., van, Hattem JM, Storm-Versloot, M.N., Ubbink, D.T., Westerbos, S.J, Topical silver for treating infected wounds. Cochrane Database System. Rev., 2007, 10.1002/14651858.CD005486.pub2.
Barku V.Y.A., Boye A., Quansah N. Antioxidant and wound healing studies on the extracts of Corchorus olitorius leaf2013;1:67–73.
Thioune, O., Khouma, B., Diarra, M., Diop, A.B., Lô, I, [The excipient properties of shea butter compared with vaseline and lanolin]. J. Pharm. Belg. 58 (2003), 81–84.
Verma, N., Chakrabarti, R., Das, R.H., Gautam, H.K, Anti-inflammatory effects of shea butter through inhibition of iNOS, COX-2, and cytokines via the NF-κb pathway in LPS-activated J774 macrophage cells. J. Complement. Integr. Med., 9, 2012 Article 410.1515/1553-38401574.