[en] The lack of robust methods to preserve, purify and in vitro maintain the phenotype of the human liver’s highly specialized parenchymal and non-parenchymal cell types importantly hampers their exploitation for the development of research and clinical applications. There is in this regard a growing interest in the use of tissue-specific extracellular matrix (ECM) to provide cells with an in vitro environment that more closely resembles that of the native tissue. In the present study, we have developed a method that allows for the isolation and downstream application of the human liver’s main cell types from cryopreserved material. We also isolated and solubilized human liver ECM (HL-ECM), analyzed its peptidomic and proteomic composition by mass spectrometry and evaluated its interest for the culture of distinct primary human liver cells. Our analysis of the HL-ECM revealed proteomic diversity, type 1 collagen abundance and partial loss of integrity following solubilization. Solubilized HL-ECM was evaluated either as a coating or as a medium supplement for the culture of human primary hepatocytes, hepatic stellate cells and liver sinusoidal endothelial cells. Whereas the solubilized HL-ECM was suitable for cell culture, its impact on the phenotype and/or functionality of the human liver cells was limited. Our study provides a first detailed characterization of solubilized HL-ECM and a first report of its influence on the culture of distinct human primary liver cells.
Alevra Sarika, Niki ✱; Université catholique de Louvain (UCLouvain) > Institute of Experimental and Clinical Research (IREC) > Laboratory of Pediatric Hepatology and Cell Therapy (PEDI)
Payen, Valéry ✱; Université catholique de Louvain (UCLouvain) > Institute of Experimental and Clinical Research (IREC) > Laboratory of Pediatric Hepatology and Cell Therapy (PEDI)
Ravau, Joachim; Université catholique de Louvain (UCLouvain) > Institute of Experimental and Clinical Research (IREC) > Laboratory of Pediatric Hepatology and Cell Therapy (PEDI)
Brusa, Davide; Université Catholique de Louvain (UCLouvain) > Institute of Experimental and Clinical Research (IREC) > Flow Cytometry Technological Platform
Najimi, Mustapha; Université catholique de Louvain (UCLouvain) > Institute of Experimental and Clinical Research (IREC) > Laboratory of Pediatric Hepatology and Cell Therapy (PEDI)
De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Eppe, Gauthier ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Mazzucchelli, Gabriel ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Sokal, Etienne; Université catholique de Louvain (UCLouvain) > Institute of Experimental and Clinical Research (IREC) > Laboratory of Pediatric Hepatology and Cell Therapy (PEDI)
des Rieux, Anne; Université catholique de Louvain (UCLouvain) > Louvain Drug Research Institute (LDRI) > Laboratory of Advanced Drug Delivery and Biomaterials (ADDB)
El Taghdouini, Adil; Université catholique de Louvain (UCLouvain) > Institute of Experimental and Clinical Research (IREC) > Laboratory of Pediatric Hepatology and Cell Therapy (PEDI)
✱ These authors have contributed equally to this work.
Language :
English
Title :
Human Liver-Derived Extracellular Matrix for the Culture of Distinct Human Primary Liver Cells
Publication date :
30 May 2020
Journal title :
Cells
eISSN :
2073-4409
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI), Basel, Switzerland
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