[en] In order to better understand the host-fungus relationship in Microsporum canis dermatophytosis and to identify major fungal antigens, the immune response to a crude exoantigen preparation and to a purified recombinant keratinolytic metalloprotease (r-MEP3) was evaluated in guinea pigs experimentally infected with M. canis. Humoral and cellular immune responses were assessed from day 0 to day 57 post-infection (PI), the former by enzyme-linked immunosorbent assay (ELISA) and the latter via a lymphocyte proliferation assay. Infected guinea pigs developed humoral and cellular responses to both M. canis exoantigen and r-MEP3, while no specific immune response to these antigens was observed in control animals. This is the first report on the development of both humoral and cell-mediated immune responses to a purified keratinase in M. canis dermatophytosis.
Disciplines :
Veterinary medicine & animal health Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Brouta, Frédéric
Descamps, Frédéric
Vermout, Sandy
Monod, Michel
Losson, Bertrand ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Mignon, Bernard ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Language :
English
Title :
Humoral and cellular immune response to a Microsporum canis recombinant keratinolytic metalloprotease (r-MEP3) in experimentally infected guinea pigs
Apodaca G, McKerrow JH. Purification and characterization of a 27,000-Mr extracellular proteinase from Trichophyton rubrum. Infect Immun 1989; 57: 3072-3080.
Mignon BR, Leclipteux T, Focant C, Nikkels AJ, Piérard GE, Losson BJ. Humoral and cellular immune response to a crude exoantigen and purified keratinase of Microsporum canis in experimentally infected guinea pigs. Med Mycol 1999; 37: 123-129.
Lee KH, Lee JB, Lee MG, Song DH. Detection of circulating antibodies to purified keratinolytic proteinase in sera from guinea pigs infected with Microsporum canis by enzyme-linked immunosorbent assay. Arch Dermatol Res 1988; 280: 45-49.
Lee KH, Yang AK, Lee MG, Lee JB. Detection of IgG and IgM antibodies to purified keratinolytic proteinase in sera from patients with dermatophytosis by enzyme-linked immunosorbent assay. Ann Dermatol 1989; 1: 1-5.
Mignon BR, Coignoul F, Leclipteux T, Focant C, Losson BJ. Histopathological pattern and humoral immune response to a crude exoantigen and purified keratinase of Microsporum canis in symptomatic and asymptomatic infected cats. Med Mycol 1999; 37: 1-9.
Mignon BR, Nikkels AF, Pierard GE, Losson BJ. The in vitro and in vivo production of a 31.5-kD keratinolytic subtilase from Microsporum canis and the clinical status in naturally infected cats. Dermatology 1998; 196: 438-441.
Lee KH, Park HW, Lee JB. Detection of keratinolytic proteinase in skin tissues from guinea pigs infected with Microsporum canis by an immunoperoxidase technique. J Dermatol Sci 1990; 1: 447-453.
Brouta F, Descamps F, Monod M, Vermout S, Losson B, Mignon B. Secreted metalloprotease gene family of Microsporum canis. Infect Immun 2002; 70: 5676-5683.
Descamps F, Brouta F, Monod M, Zaugg C, Baar D, Losson B, Mignon B. Isolation of a Microsporum canis gene family encoding three subtilisin-like proteases expressed in vivo. J Invest Dermatol 2002; 119: 830-835.
Brouta F, Descamps F, Fett T, Losson B, Gerday C, Mignon B. Purification and characterization of a 43.5 kDa keratinolytic metalloprotease from Microsporum canis. Med Mycol 2001; 39: 269-275.
Van Cutsem J. Animal models for dermatomycotic infections. Curr Top Med Mycol 1989; 3: 1-35.
Mignon B, Swinnen M, Bouchara JP, et al. Purification and characterization of a 31.5 kDa keratinolytic subtilisin-like serine protease from Microsporum canis and evidence of its secretion in naturally infected cats. Med Mycol 1998; 36: 395-404.
Grappel SF, Blank F. Role of keratinases in dermatophytosis. I. Immune responses of guinea pigs infected with Trichophyton mentagrophytes and guinea pigs immunized with keratinases. Dermatologica 1972; 145: 245-255.
Sparkes AH, Stokes CR, Gruffydd-Jones TJ. Experimental Microsporum canis infection in cats: Correlation between immunological and clinical observations. J Med Vet Mycol 1995; 33: 177-184.
Sparkes AH, Stokes CR, Gruffydd-Jones TJ. Humoral immune responses in cats with dermatophytosis. Am J Vet Res 1993; 54: 1869-1873.
DeBoer DJ, Moriello KA. Humoral and cellular immune responses to Microsporum canis in naturally occurring feline dermatophytosis. J Med Vet Mycol 1993; 31: 121-132.
Cox WA, Moore JA. Experimental Trichophyton verrucosum infections in laboratory animals. J Comp Pathol 1968; 78: 35-41.
Kristensen F, Kristensen B, Lazary S. The lymphocyte stimulation test in veterinary immunology. Vet Immunol Immunopathol 1982; 3: 203-277.
Sohnle PG. Dermatophytosis. In: Cox RA (ed.). Immunology of the Fungal Diseases. Boca Raton, FL: CRC Press, 1989; 1-27.
Svejgaard E. Immunologic investigations of dermatophytes and dermatophytosis. Seminars in Dermatology 1985; 4: 201-221.
Jones HE, Reinhardt JH, Rinaldi MG. Acquired immunity to dermatophytes. Arch Dermatol 1974; 109: 840-848.
Jones HE, Reinhardt JH, Rinaldi MG. Model dermatophytosis in naturally infected subjects. Arch Dermatol 1974; 110: 369-374.
Jones HE, Reinhardt JH, Rinaldi MG. A clinical, mycological, and immunological survey for dermatophytosis. Arch Dermatol 1973; 108: 61-65.
Hay RJ. Immunomodulation in superficial fungal infections. In: Jacobs PH, Nall L (eds). Fungal Disease. Biology, Immunology and Diagnosis. New York: Marcel Dekker, 1997: 209-218.
Calderon RA, Hay RJ. Cell-mediated immunity in experimental murine dermatophytosis. II. Adoptive transfer of immunity to dermatophyte infection by lymphoid cells from donors with acute or chronic infections. Immunology 1984; 53: 465-472.
DeBoer DJ, Moriello KA, Cooley AJ. Immunological reactivity to intradermal dermatophyte antigens in cats with dermatophytoses. Vet Dermatol 1991; 2: 59-67.
Wawrzkiewicz K, Ziolkowska G, Wawrzkiewicz J. An evaluation of the resistance to Microsporum canis on the basis of the guinea pig. Arch Vet Pol 1994; 34: 153-162.
Calderon RA, Shennan GI. Susceptibility of Trichophyton quinckeanum and Trichophyton rubrum to products of oxidative metabolism. Immunology 1987; 61: 283-288.
King RD, Khan HA, Foye JC, Greenberg JH, Jones HE. Transferrin, iron, and dermatophytes. I. Serum dematophyte inhibitory component definitively identified as unsaturated transferrin. J Lab Clin Med 1975; 86: 204-212.
Carlisle DH, Inouye JC, King RD, Jones HE. Significance of serum fungal inhibitory factor in dermatophytosis. J Invest Dermatol 1974; 63: 239-241.
Yu RJ, Grappel SF, Blank F. Inhibition of keratinases by 2-macroglobulin. Experientia 1972; 28: 886.
Berk SH, Penneys NS, Weinstein GD. Epidermal activity in annular dermatophytosis. Arch Dermatol 1976; 112: 485-488.
Tagami H. Epidermal cell proliferation in guinea pigs with experimental dermatophytosis. J Invest Dermatol 1985; 85: 153-155.