Monitoring changes in plasma levels of pancreatic and intestinal enzymes in a model of pancreatic exocrine insufficiency–induced by pancreatic duct-ligation–in young pigs
[en] Purpose: Plasma levels of pancreatic and intestinal enzymes were measured after pancreatic duct ligation (PDL) to monitor pancreatic exocrine insufficiency (PEI) in a model using young pigs.
Material/methods: Five, 6 week-old pigs (10.9±0.2kg), underwent PDL while age-matched, un-operated pigs were used as controls. Plasma levels of immunoreactive cationic trypsinogen (IRCT), amylase, lipase, and diamine oxidase (DAO) activities were analyzed for 48 days after PDL, including 1 week of oral pancreatic enzyme supplementation (PES) with Creon(®).
Results: PDL resulted in an arrested body growth and a rapid surge of pancreatic enzymes (IRCT, amylase and lipase) into the plasma. Nine days after PDL, the plasma levels of these pancreatic enzymes had decreased. IRCT then remained below the level in un-operated pigs while amylase only fell below control at 25 days. The intestinally derived marker DAO and plasma protein levels were unaffected by PDL but DAO decreased slightly with time in PEI pigs. One-week of oral PES restored body growth, but had little effect on pancreatic enzyme plasma levels, except for a tendency towards increased DAO.
Conclusions: The study showed that PEI developed within 1-2 weeks after PDL and that only IRCT is a reliable plasma enzyme marker for this. The reduced plasma DAO indicated that PEI also affected the intestines, while PES therapy restored growth of the PDL pigs and slightly increased plasma DAO, suggesting an improved intestinal function.
Disciplines :
Gastroenterology & hepatology
Author, co-author :
Lozinska, L.
Prykhodko, O.
Arevalo Sureda, Ester ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Ingénierie des productions animales et nutrition
Szwiec, K.
Podgurniak, P.
Pierzynowski, S.
Weström, B.
Language :
English
Title :
Monitoring changes in plasma levels of pancreatic and intestinal enzymes in a model of pancreatic exocrine insufficiency–induced by pancreatic duct-ligation–in young pigs
Abrams CK, Hamosh M, Hubbard VS, Dutta SK, Hamosh P. Lingual lipase in cystic fibrosis. Quantitation of enzyme activity in the upper small intestine of patients with exocrine pancreatic insufficiency. J Clin Investig 1984;73(2):374-82. http://dx.doi.org/10.1172/jci111222 [PubMed PMID: 6699170; PubMed Central PMCID: PMCPmc425027].
Aggett PJ, Cavanagh NP, Matthew DJ, Pincott JR, Sutcliffe J, Harries JT. Shwachman's syndrome. A review of 21 cases. Arch Dis Child 1980;55(5):331-47 [PubMed PMID: 7436469; PubMed Central PMCID: PMCPmc1626878].
Aghdassi AA, Mayerle J, Christochowitz S, Weiss FU, Sendler M, Lerch MM. Animal models for investigating chronic pancreatitis. Fibrogenesis Tissue Repair 2011;4(1):26. http://dx.doi.org/10.1186/1755-1536-4-26 [PubMed PMID: 22133269; PubMed Central PMCID: PMC3274456].
Fedkiv O, Rengman S, Westrom BR, Pierzynowski SG. Growth is dependent on the exocrine pancreas function in young weaners but not in growing-finishing pigs. J Physiol Pharmacol 2009;60(Suppl. 3):55-9 [PubMed PMID: 19996482].
Rengman S, Fedkiv O, Botermans J, Svendsen J, Westrom B, Pierzynowski S. An elemental diet fed, enteral or parenteral, does not support growth in young pigs with exocrine pancreatic insufficiency. Clin Nutr (Edinb Scotl) 2009;28(3):325-30 [PubMed PMID: 19297056].
Abello J, Pascaud X, Simoes-Nunes C, Cuber JC, Junien JL, Roze C. Total pancreatic insufficiency in pigs: a model to study intestinal enzymes and plasma levels of digestive hormones after pancreatic supplementation by a whole pancreas preparation. Pancreas 1989;4(5):556-64 [PubMed PMID: 2478998].
Prykhodko O, Fedkiv O, Weström BR, Pierzynowski SG. Effects on gut properties in exocrine pancreatic insufficient (EPI) pigs, being growth retarded due to pancreatic duct ligation at 7 weeks but not at 16 weeks of age. Adv Med Sci 2014;59(1):74-80.
Apple F, Benson P, Preese L, Eastep S, Bilodeau L, Heiler G. Lipase and pancreatic amylase activities in tissues and in patients with hyperamylasemia. Am J Clin Pathol 1991;96(5):610-4 [PubMed PMID: 1719798].
Hussain M, Dandona P, Fedail SS, Ramdial L, Flynn D, Hoffbrand AV. Serum immu-noreactive trypsin in beta-thalassaemia major. J Clin Pathol 1981;34(9):970-1 [PubMed PMID: 7276222; PubMed Central PMCID: PMCPmc494207].
Sandström JP, Weström BR, Pierzynowski SG, Ohlsson BG, Karlsson BW. Immunoreactive trypsin in blood serum as a marker of pancreatic function and development in the young pig. In: Proc 9th Int Pig Vet Soc Congr July 15-18. (Abstr.), Barcelona, Spain. 1986. p. 468.
Luk GD, Bayless TM, Baylin SB. Diamine oxidase (histaminase). A circulating marker for rat intestinal mucosal maturation and integrity. J Clin Investig 1980;66(1):66-70. http://dx.doi.org/10.1172/jci109836 [PubMed PMID: 6772669; PubMed Central PMCID: PMCPmc371506].
Rantzer D, Svendsen J, Westrom B. Weaning of pigs raised in sow-controlled and in conventional housing systems: 1. Description of systems, production, and bacteriology. Swed J Agric Res 1995;25:37-46.
Gewert K, Holowachuk SA, Rippe C, Gregory PC, Erlanson-Albertsson C, Olivecrona G, et al. The enzyme levels in blood are not affected by oral administration of a pancreatic enzyme preparation (Creon 10,000) in pancreasinsufficient pigs. Pancreas 2004;28(1):80-8 [PubMed PMID: 14707735].
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193(1):265-75 [PubMed PMID: 14907713].
Bai JC. Malabsorption syndromes. Digestion 1998;59(5):530-46.
Pencharz PB, Durie PR. Pathogenesis of malnutrition in cystic fibrosis, and its treatment. Clin Nutr (Edinb Scotl) 2000;19(6):387-94. http://dx.doi.org/10.1054/clnu.1999.0079 [PubMed PMID: 11104588].
Saloniemi H, Kalima TV, Rahko T. Pancreatic enzyme supplementation in normal and exocrine pancreatic insufficient pigs. Acta Vet Scand 1989;30(4):367-70 [PubMed PMID: 2484259].
Kloppel G, Maillet B. Pseudocysts in chronic pancreatitis: a morphological analysis of 57 resection specimens and 9 autopsy pancreata. Pancreas 1991;6(3):266-74 [PubMed PMID: 1862065].
Gorelick FS. Diabetes mellitus and the exocrine pancreas. Yale J Biol Med 1983;56(4):271-5 [PubMed PMID: 6367237; PubMed Central PMCID: PMCPmc2589620].
Dossin O. Laboratory tests for diagnosis of gastrointestinal and pancreatic diseases. Top Companion Anim Med 2011;26(2):86-97. http://dx.doi.org/10.1053/j.tcam.2011.02.005 [PubMed PMID: 21596348].
Yadav D, Agarwal N, Pitchumoni CS. A critical evaluation of laboratory tests in acute pancreatitis. Am J Gastroenterol 2002;97(6):1309-18. http://dx.doi.org/10.1111/j. 1572-0241.2002.05766.x [PubMed PMID: 12094843].
Terada T, Morita T, Hoso M, Nakanuma Y. Pancreatic enzymes in the epithelium of intrahepatic large bile ducts and in hepatic bile in patients with extrahepatic bile duct obstruction. J Clin Pathol 1994;47(10):924-7 [PubMed PMID: 7962606; PubMed Central PMCID: PMCPmc502177].
Kanno N, LeSage G, Glaser S, Alvaro D, Alpini G. Functional heterogeneity of the intrahepatic biliary epithelium. Hepatology (Baltimore MD) 2000;31(3):555-61. http://dx.doi.org/10.1002/hep.510310302 [PubMed PMID: 10706542].
Boerma D, Straatsburg IH, Offerhaus GJ, Gouma DJ, van Gulik TM. Experimental model of obstructive, chronic pancreatitis in pigs. Dig Surg 2003;20(6):520-6 [DOI: 73688. PubMed PMID: 14534374].
Suchodolski JS, Steiner JM. Laboratory assessment of gastrointestinal function. Clin Tech Small Anim Pract 2003;18(4):203-10. http://dx.doi.org/10.1016/s1096-2867(03)00075-6 [PubMed PMID: 14738200].
Kamei H, Hachisuka T, Nakao M, Takagi K. Quick recovery of serum diamine oxidase activity in patients undergoing total gastrectomy by oral enteral nutrition. Am Journal Surg 2005;189(1):38-43.
Donaldson J, Fed'kiv O, Pawlowska M, Kowalik S, Erlwanger KH, Westrom B, et al. The effectiveness of enzymatic replacement therapy measured by turbidimetry and the lipaemic index in exocrine pancreatic insufficient young, growing pigs, fed a high-fat diet. Adv Med Sci 2009;54(1):7-13 [PubMed PMID: 19648084].
KA A, Saleh SY, El-hamidSafaa SA, ZakiAmal A, Mohamed AE-S. Effects of exogenous multi-enzyme feed additive (kemzyme) on the activities of certain digestive enzymes and intestinal morphology in growing rabbits. J Agric Sci 2011;4(3):35-44. http://dx.doi.org/10.5539/jas.v4n3p35.