Article (Scientific journals)
Identification and modulation of a growth hormone-binding protein in rainbow trout (Oncorhynchus mykiss) plasma during seawater adaptation.
Sohm, F.; Manfroid, Isabelle; Pezet, A. et al.
1998In General and Comparative Endocrinology, 111 (2), p. 216-24
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Keywords :
Adaptation, Physiological; Animals; Binding, Competitive; Carrier Proteins/metabolism; Chromatography, High Pressure Liquid; Fish Proteins; Glycoproteins/metabolism; Growth Hormone/metabolism; Iodine Radioisotopes; Oncorhynchus mykiss/blood; Pituitary Hormones/metabolism; Precipitin Tests; Receptors, Somatotropin/metabolism; Recombinant Proteins; Seawater
Abstract :
[en] A soluble protein that specifically bound 125I-human growth hormone (hGH) was identified in rainbow trout plasma, using HPLC-gel filtration. The binding affinity of the protein for hGH was 1.2 x 10(9)M-1. 125I-rainbow trout GH (tGH) was also able to bind to the protein albeit with a lower affinity (6.6 x 10(7)M-1) than hGH. Crosslinking experiments using 125I-hGH revealed two specific bands of 150 and 130 kDa. The complex 125I-hGH-BP could be precipitated by a monoclonal anti-GH receptor antibody, suggesting a close relationship between the plasma GH-BP and the GH receptor. A fourfold increase in the hGH binding to the GH-BP was shown 48 h after transfer of the fishes from freshwater to seawater. The increase in binding was related to a high binding capacity without significant changes in binding affinity. These results suggest a potential role of this related GH-BP as an index of GH effects during seawater adaptation in salmonids.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Sohm, F.
Manfroid, Isabelle ;  Université de Liège - ULiège > GIGA-Research
Pezet, A.
Rentier-Delrue, Françoise ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire - GIGA-R : Coordination scientifique
Rand-Weaver, M.
Kelly, P. A.
Boeuf, G.
Postel-Vinay, M. C.
de Luze, A.
Edery, M.
Language :
English
Title :
Identification and modulation of a growth hormone-binding protein in rainbow trout (Oncorhynchus mykiss) plasma during seawater adaptation.
Publication date :
1998
Journal title :
General and Comparative Endocrinology
ISSN :
0016-6480
eISSN :
1095-6840
Publisher :
Elsevier, New York, United States - New York
Volume :
111
Issue :
2
Pages :
216-24
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright 1998 Academic Press.
Available on ORBi :
since 09 February 2010

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