Bacillus subtilis; flagellin; in-gel digest; mass spectrometry; PF2D; proteomics
Abstract :
[en] We are confronted daily to unknown microorganisms that have yet to be characterized, detected, and/ or analyzed. We propose, in this study, a multidimensional strategy using polyclonal antibodies, consisting of a novel proteomic tool, the ProteomeLab PF2D, coupled to immunological techniques and mass spectrometry ( i-PF2D-MS/MS). To evaluate this strategy, we have applied it to Bacillus subtilis, considered here as our unknown bacterial model.
Research Center/Unit :
Giga-Systems Biology and Chemical Biology - ULiège CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Ruelle, Virginie ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Falisse-Poirier, Nandini
Elmoualij, Benaïssa ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Zorzi, Danièle ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Pierard, Olivier ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Heinen, Ernst ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
De Pauw, Edwin ; Université de Liège - ULiège > Chimie physique, spectrométrie de masse
Zorzi, Willy ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Language :
English
Title :
An immuno-PF2D-MS/MS proteomic approach for bacterial antigenic characterization: To Bacillus and beyond
Publication date :
June 2007
Journal title :
Journal of Proteome Research
ISSN :
1535-3893
eISSN :
1535-3907
Publisher :
Amer Chemical Soc, Washington, United States - Washington
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