Article (Scientific journals)
Experimental Usutu Virus Infection in Domestic Canaries Serinus canaria.
Benzarti, Emna; Rivas Troncoso, José Felipe Antonio; Sarlet, Michaël et al.
2020In Viruses, 12 (2)
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Keywords :
Usutu virus; domestic canaries; experimental infection
Abstract :
[en] Usutu virus (USUV) is a neurotropic flavivirus closely related to West Nile virus (WNV). Its enzootic cycle mainly involves mosquitoes and birds. Human infection can occur with occasional, but sometimes severe, neurological complications. Since its emergence and spread in Europe over the last two decades, USUV has been linked to significant avian outbreaks, especially among Passeriformes, including European blackbirds (Turdus merula). Strikingly, no in vivo avian model exists so far to study this arbovirus. The domestic canary (Serinus canaria) is a passerine, which is considered as a highly susceptible model of infection by WNV. Here, we experimentally challenged domestic canaries with two different doses of USUV. All inoculated birds presented detectable amounts of viral RNA in the blood and RNA shedding via feathers and droppings during the early stages of the infection, as determined by RT-qPCR. Mortality occurred in both infected groups (1/5 and 2/5, respectively) and was not necessarily correlated to a pure neurological disease. Subsequent analyses of samples from dead birds showed histopathological changes and virus tropism mimicking those reported in naturally infected birds. A robust seroconversion followed the infection in almost all the surviving canaries. Altogether, these results demonstrate that domestic canaries constitute an interesting experimental model for the study of USUV pathogenesis and transmission.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Benzarti, Emna ;  Université de Liège - ULiège > FARAH
Rivas Troncoso, José Felipe Antonio ;  Université de Liège - ULiège > FARAH
Sarlet, Michaël ;  Université de Liège - ULiège > Département de morphologie et pathologie (DMP) > Département de morphologie et pathologie (DMP)
Franssen, Mathieu 
Desmecht, Daniel ;  Université de Liège - ULiège > Département de morphologie et pathologie (DMP) > Pathologie spéciale et autopsies
Schmidt-Chanasit, Jonas
Savini, Giovanni
Lorusso, Alessio
Van Laere, Anne-Sophie  ;  Université de Liège - ULiège > Département de morphologie et pathologie (DMP) > Pathologie spéciale et autopsies
Garigliany, Mutien-Marie  ;  Université de Liège - ULiège > Département de morphologie et pathologie (DMP) > Pathologie générale et autopsies
Language :
English
Title :
Experimental Usutu Virus Infection in Domestic Canaries Serinus canaria.
Publication date :
2020
Journal title :
Viruses
eISSN :
1999-4915
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI), Switzerland
Volume :
12
Issue :
2
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 15 May 2020

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