Molecular strategies for adapting Bacillus subtilis 168 biosurfactant production to biofilm cultivation mode

Bacillus subtilis; Biofilm bioreactor; Cell adhesion; Filamentation; Surfactin; Bacteriology; Bioconversion; Biofilms; Bioreactors; Cell immobilization; Cell proliferation; Cells; Genetic engineering; Metabolites; Adhesion capacities; Biosurfactant production; Exopolysaccharides; Microbial cell factories; Time-lapse microscopy; Cell engineering; Article; Lipopeptides; Peptides, Cyclic

Abstract :

[en] Biofilm bioreactors have already been proven to be efficient systems for microbial lipopeptide production since they avoid foam formation. However, the cell adhesion capacities of the laboratory strain B. subtilis 168 to the biofilm bioreactor support are limited. In this work, we present a novel approach for increasing cell adhesion through the generation of filamentous and/or exopolysaccharide producing B. subtilis 168 mutants by genetic engineering. The single cell growth behavior was analyzed using time-lapse microscopy and the colonization capacities were investigated under continuous flow conditions in a drip-flow reactor. Cell adhesion could be increased three times through filamentous growth in lipopeptide producing B. subtilis 168 derivatives strains. Further restored exopolysaccharide production increased up to 50 times the cell adhesion capacities. Enhanced cell immobilization resulted in 10 times increased surfactin production. These findings will be of particular interest regarding the design of more efficient microbial cell factories for biofilm cultivation. © 2019 Elsevier Ltd

Disciplines :

Biotechnology

Author, co-author :

Brück, Hannah ; Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies

Delvigne, Frank ; Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies

Dhulster, Pascal; Univ. Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 ICV – Institut Charles Viollette, Lille, F-59000, France

Jacques, Philippe ; Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies

Coutte, François; Univ. Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 ICV – Institut Charles Viollette, Lille, F-59000, France

Language :

English

Title :

Molecular strategies for adapting Bacillus subtilis 168 biosurfactant production to biofilm cultivation mode

Publication date :

2019

Journal title :

Bioresource Technology

ISSN :

0960-8524

eISSN :

1873-2976

Publisher :

Elsevier Ltd

Volume :

293

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

ULiège - Université de Liège [BE]

Available on ORBi :

since 14 May 2020

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