Evaluation of seven commercial African swine fever virus detection kits and three Taq polymerases on 300 well-characterized field samples

Schoder, M.-E.; Tignon, M.; Linden, Annick; Vervaeke, M.; Cay, A. B.

doi:10.1016/j.jviromet.2020.113874

Article (Scientific journals)

Evaluation of seven commercial African swine fever virus detection kits and three Taq polymerases on 300 well-characterized field samples

Schoder, M.-E.; Tignon, M.; Linden, Annick et al.

2020 • In Journal of Virological Methods, 280

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/247419

DOI
10.1016/j.jviromet.2020.113874

PubMed
32360149

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Keywords :

African swine fever; Real-time PCR; Wild boar

Abstract :

[en] African swine fever virus (ASFV) is a complex double stranded DNA virus, responsible for a highly infectious and fatal disease in pigs and boars and for important deterioration of animal welfare. Over the last decade, the disease spread to several European and Asian countries causing unprecedented dramatic economic losses in pig industry. In the absence of a vaccine, affected countries rely on trustful diagnostic tests and adapted testing policies to set up control programs to fight against the disease. In this study, we evaluated the sensitivity and specificity of seven commercially available ASFV real-time PCR detection kits and three Taq polymerases on 300 well-characterized wild boar samples collected in Belgium during the 2018–2019 outbreak. This study confirms that all commercial kits and two Taq polymerases are suitable for ASFV detection in diagnostic laboratories. Furthermore, the use of endogenous controls is emphasized when testing field samples harvested on carcasses in an advanced stage of decomposition, in order to avoid false negative results. © 2020 Elsevier B.V.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Schoder, M.-E.; Sciensano, Directorate of Infectious diseases in animals, Service of Enzootic, vector-borne and bee diseases, 99 Groeselenbergstraat, Brussels, 1180, Belgium

Tignon, M.; Sciensano, Directorate of Infectious diseases in animals, Service of Enzootic, vector-borne and bee diseases, 99 Groeselenbergstraat, Brussels, 1180, Belgium

Linden, Annick ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Santé et pathologies de la faune sauvage

Vervaeke, M.; Agentschap voor Natuur en Bos (ANB), Havenlaan 88 bus 75, Brussels, 1000, Belgium

Cay, A. B.; Sciensano, Directorate of Infectious diseases in animals, Service of Enzootic, vector-borne and bee diseases, 99 Groeselenbergstraat, Brussels, 1180, Belgium

Language :

English

Title :

Evaluation of seven commercial African swine fever virus detection kits and three Taq polymerases on 300 well-characterized field samples

Publication date :

2020

Journal title :

Journal of Virological Methods

ISSN :

0166-0934

eISSN :

1879-0984

Publisher :

Elsevier B.V.

Volume :

280

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

ANB - Agentschap Natuur en Bos [BE]

Available on ORBi :

since 13 May 2020

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