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Optimization of biosurfactant production in a trickle-bed biofilm reactor with genetically improved bacteria
Brück, Hannah; Coutte, François; Delvigne, Frank et al.
202025th National Symposium for Applied Biological Science (NSABS)
 

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Keywords :
Surfactin; Biofilm bioreactor; Bacillus subtilis
Abstract :
[en] The Gram-positive soil bacterium Bacillus subtilis is a potential producer of the very powerful biosurfactant surfactin [1]. In the phytosanitary field, surfactin gains increasing attention for the use as biocontrol agent since it is able to induce systemic resistance in plants [1]. Furthermore, surfactin has many applications in different industrial sectors due to the exceptional foaming and emulsifying properties [1]. Biofilm bioreactors are efficient alternative production systems for bacterial biosurfactants since excessive foam formation can be avoided while a high air/liquid mass transfer can be obtained [2-5]. The widely used B. subtilis 168 strain has very low cell adhesion capacities and thus a reduced growth in biofilm-based systems. In this work, genetically modified B. subtilis 168 mutants have been designed to improve the natural cell immobilization via biofilm formation. The mutants were first cultivated in a drip-flow biofilm reactor to assess their biofilm formation capacities [6]. Then, the strains with the best performances were selected for the cultivation in a trickle-bed biofilm reactor. The mutants with functional biofilm matrix production showed a significantly improved adhesion capacity compared to the control strain as well as an improved surfactin productivity. The introduced cell filamentation seemed to improve cell cohesion and to decrease cell detachment.
Disciplines :
Biotechnology
Author, co-author :
Brück, Hannah ;  Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies
Coutte, François;  Université de Lille > Institut Charles Viollette
Delvigne, Frank  ;  Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies
Dhulster, Pascal;  Université de Lille > Institut Charles Viollette
Jacques, Philippe ;  Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies
Language :
English
Title :
Optimization of biosurfactant production in a trickle-bed biofilm reactor with genetically improved bacteria
Publication date :
2020
Event name :
25th National Symposium for Applied Biological Science (NSABS)
Event place :
Gembloux, Belgium
Event date :
31-01-2020
Funders :
Région Hauts-de-France [FR]
ULiège - Université de Liège [BE]
Available on ORBi :
since 08 May 2020

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