[en] Puumala hantavirus (PUUV) sequences were recovered from red bank voles (Clethrionomys glareolus) trapped between 1996 and 1998 in four localities of southern Belgium: Thuin, Montbliart, Momignies and Couvin. In addition, three PUUV isolates originating from bank voles trapped in the 1980s in southern (Montbliart) and northern (Turnhout) Belgium were genetically characterized. Analysis of the complete S and partial M segment sequences showed that the Belgian PUUV strains constitute a genetic lineage, distinct from other known PUUV lineages from Europe and Japan. This lineage also includes a wild strain (Cg-Erft) originating from a neighbouring area of Germany. Within the Belgian lineage, geographical clustering of genetic variants was observed. In the Montbliart site, the range of diversity between the most temporally distant strains (from 1986 and 1996-1998) was higher than between those from 1996 and 1998, suggesting slight genetic drift via accumulation of neutral or quasi-neutral substitutions with time.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Escutenaire, Sophie; Université de Liège - ULiège > Faculty of Veterinary Medicine / Immunology - Vaccinology
Chalon, Patrice ; Université de Liège - ULiège > Faculty of Veterinary Medicine / Immunology - Vaccinology
Heyman, Paul; Queen Astrid Military Hospital > Research Laboratory for Vector-borne Diseases
Van der Auwera, Gert; Institute of Tropical Medicine > Microbiology
van der Groen, Guido; Institute of Tropical Medicine > Microbiology
Verhagen, Ron; RUCA > Evolutionary Biology
Thomas, Isabelle
Karelle-Bui, Lucie; Université de Liège - ULiège > Faculty of Veterinary Medicine / Immunology - Vaccinology
Vaheri, Antti; University of Helsinki > Haartman Institute > Virology
Pastoret, Paul-Pierre ; Université de Liège - ULiège > Faculty of Veterinary Medicine / Immunology - Vaccinology
Plyusnin, Alexander; University of Helsinki > Haartman Institute > Virology
Language :
English
Title :
Genetic Characterization of Puumala Hantavirus Strains from Belgium : evidence for a Distinct Phylogenetic Lineage
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