Differential effects of inulin or its fermentation metabolites on gut barrier and immune function of porcine intestinal epithelial cells

Uerlings, Julie; Schroyen, Martine; Willems, Els; Tanghe, Sofie; Bruggeman, Geert; Bindelle, Jérôme; Everaert, Nadia

doi:10.1016/j.jff.2020.103855

Article (Scientific journals)

Differential effects of inulin or its fermentation metabolites on gut barrier and immune function of porcine intestinal epithelial cells

Uerlings, Julie; Schroyen, Martine; Willems, Els et al.

2020 • In Journal of Functional Foods

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/246332

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10.1016/j.jff.2020.103855

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Abstract :

[en] Prebiotics can modulate gut fermentation and improve intestinal barrier function in mammals. First, inulin fermentation profile was tested in a three-step in vitro model of the piglet’s gastro-intestinal tract combining a hydrolysis – dialysis step to a batch fermentation. Then, the differential effects of digested inulin (after the hydrolysis – dialysis steps) or fermented inulin (after the fermentation step) on the expression of gut barrier and immune-related genes of IPEC-J2 cells were investigated by high-throughput qPCR. Inulin was associated with elevated short-chain fatty acids and butyrate levels. Upregulated expressions of tight and adherens junction genes were observed in IPEC-J2 cells supplemented with inulin fermentation supernatant compared to control IPEC-J2 cells and digested inulin. Therefore, metabolites arising from the fermentation process, including butyrate, could be responsible for the reinforcement of the barrier function.

Disciplines :

Animal production & animal husbandry

Author, co-author :

Uerlings, Julie ; Université de Liège - ULiège > Département GxABT > Ingénierie des productions animales et nutrition

Schroyen, Martine ; Université de Liège - ULiège > Département GxABT > Département GxABT

Willems, Els; Royal Agrifirm Group

Tanghe, Sofie; Royal Agrifirm Group

Bruggeman, Geert; Royal Agrifirm Group

Bindelle, Jérôme ; Université de Liège - ULiège > Département GxABT > Ingénierie des productions animales et nutrition

Everaert, Nadia ; Université de Liège - ULiège > Département GxABT > Ingénierie des productions animales et nutrition

Language :

English

Title :

Differential effects of inulin or its fermentation metabolites on gut barrier and immune function of porcine intestinal epithelial cells

Publication date :

2020

Journal title :

Journal of Functional Foods

ISSN :

1756-4646

eISSN :

2214-9414

Publisher :

Elsevier, Netherlands

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 31 March 2020

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