Article (Scientific journals)
Effective Temperature and Structural Rearrangement in Trapped Ion Mobility Spectrometry
Morsa, Denis; Hanozin, Emeline; Eppe, Gauthier et al.
2020In Analytical Chemistry
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Keywords :
Ion Mobility; Mass Spectrometry; TIMS; structural rearrangement; benzylpyridinium thermometer; cytochrome C
Abstract :
[en] Modern ion mobility instrumentations are typically operated above the low field limit, which may activate the ions and cause structural rearrangement or fragmentation during the analysis. Here, we quantitatively assessed the internal heating experienced by ions during trapped ion mobility spectrometry (TIMS) experiments. To this end, the fragmentation yields of fragile benzylpyridinium “thermometer” ions were monitored during both the accumulation and analysis steps inside the TIMS tunnel. The corresponding fragmentation rate constants were translated into a vibrational effective temperature Teff,vib. Our results demonstrate significant fragmentation upstream and inside the TIMS tunnel that corresponds to Teff,vib ≈ 510 K during both the accumulation and analysis steps. Broadening our scope to cytochrome c and lysozyme, we showed that although compact “native” folds can be preserved, the collision cross section distributions are highly sensitive to the transmission voltages and the analysis timescale. Our results are discussed with regard to Teff,vib data previously acquired on traveling-wave (TWIMS) ion mobility in the context of native mass spectrometry and conformational landscape exploration.
Research center :
MSLab
MolSys - Molecular Systems - ULiège
Disciplines :
Chemistry
Author, co-author :
Morsa, Denis  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Hanozin, Emeline ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Eppe, Gauthier  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Quinton, Loïc  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie biologique
Gabelica, Valérie
De Pauw, Edwin  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Language :
English
Title :
Effective Temperature and Structural Rearrangement in Trapped Ion Mobility Spectrometry
Publication date :
2020
Journal title :
Analytical Chemistry
ISSN :
0003-2700
eISSN :
1520-6882
Publisher :
American Chemical Society, United States - District of Columbia
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 February 2020

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