Effect of conventional controlled-rate freezing and vitrification on quality of bovine blasotcysts produced in vitro

[en] This study compares the effects of conventional controlled-rate freezing and vitrification on the morphology and metabolism of in vitro-produced bovine blastocysts. Day 7 expanded blastocysts cultured in synthetic oviduct fluid with 5% fetal calf serum were frozen in 1.36 M glycerol, 0.25 M sucrose or vitrified in 25% glycerol, 25% ethylene glycol. Cell alterations and in vitro development were evaluated immediately after thawing or after 72 h. The effect of cryopreservation on inner cell mass and trophectoderm (TE) cell number as well as glucose, pyruvate, and oxygen uptakes, and lactate release by blastocysts were evaluated. Immediately after thawing, blastocysts showed equivalent cell membrane permeabilization after both cryopreservation procedures, while alterations in nuclear staining were more frequent in vitrified embryos. After culture, similar survival and hatching rates were observed. Both procedures decreased cell number immediately after thawing and after 72 h. However, the number of TE cells was lower in frozen embryos than in vitrified ones. In relation to this, frozen blastocysts showed a decrease in glucose, pyruvate, and oxygen uptake, although those parameters were not altered in vitrified embryos. An increased glycolytic activity was also observed in frozen embryos, indicating a stress response to this procedure.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Kaidi, Safia

Bernard, S.

Lambert, Philippe ; Université de Liège - ULiège > Institut des sciences humaines et sociales > Méthodes quantitatives en sciences sociales

Massip, A.

Donnay, I.

Language :

English

Title :

Effect of conventional controlled-rate freezing and vitrification on quality of bovine blasotcysts produced in vitro

Publication date :

2001

Journal title :

Biology of Reproduction

ISSN :

0006-3363

eISSN :

1529-7268

Publisher :

Society for the Study of Reproduction, Madison, United States - Wisconsin

Volume :

Pages :

1127-1134

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://www.biolreprod.org/content/65/4/1127.abstract

Available on ORBi :

since 29 September 2009

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