Article (Scientific journals)
Molecular insight into cotton leaf curl geminivirus disease resistance in cultivated cotton (Gossypium hirsutum)
Syed-Shan-e-Ali, Zaïdi; Naqvi, R. Z.; Asif, M. et al.
2019In Plant Biotechnology Journal
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Keywords :
Gossypium hirsutum; WGCNA
Abstract :
[en] Cultivated cotton (Gossypium hirsutum) is the most important fibre crop in the world. Cotton leaf curl disease (CLCuD) is the major limiting factor and a threat to textile industry in India and Pakistan. All the local cotton cultivars exhibit moderate to no resistance against CLCuD. In this study, we evaluated an exotic cotton accession Mac7 as a resistance source to CLCuD by challenging it with viruliferous whiteflies and performing qPCR to evaluate the presence/absence and relative titre of CLCuD-associated geminiviruses/betasatellites. The results indicated that replication of pathogenicity determinant betasatellite is significantly attenuated in Mac7 and probably responsible for resistance phenotype. Afterwards, to decipher the genetic basis of CLCuD resistance in Mac7, we performed RNA sequencing on CLCuD-infested Mac7 and validated RNA-Seq data with qPCR on 24 independent genes. We performed co-expression network and pathway analysis for regulation of geminivirus/betasatellite-interacting genes. We identified nine novel modules with 52 hubs of highly connected genes in network topology within the co-expression network. Analysis of these hubs indicated the differential regulation of auxin stimulus and cellular localization pathways in response to CLCuD. We also analysed the differential regulation of geminivirus/betasatellite-interacting genes in Mac7. We further performed the functional validation of selected candidate genes via virus-induced gene silencing (VIGS). Finally, we evaluated the genomic context of resistance responsive genes and found that these genes are not specific to A or D sub-genomes of G. hirsutum. These results have important implications in understanding CLCuD resistance mechanism and developing a durable resistance in cultivated cotton. © 2019 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
Disciplines :
Genetics & genetic processes
Biotechnology
Author, co-author :
Syed-Shan-e-Ali, Zaïdi ;  Université de Liège - ULiège > Plant Sciences
Naqvi, R. Z.;  National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan, Boyce Thompson Institute, Ithaca, NY, United States
Asif, M.;  National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan
Strickler, S.;  Boyce Thompson Institute, Ithaca, NY, United States
Shakir, Sara  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Ingénierie des productions végétales et valorisation
Shafiq, M.;  National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan, Department of Biotechnology, University of Okara, Okara, Pakistan
Khan, A. M.;  National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan
Amin, I.;  National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan
Mishra, B.;  Department of Biology, University of Alabama at Birmingham, Birmingham, AL, United States
Mukhtar, M. S.;  Department of Biology, University of Alabama at Birmingham, Birmingham, AL, United States
Scheffler, B. E.;  Genomics and Bioinformatics Research Unit, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Stoneville, MS, United States
Scheffler, J. A.;  Crop Genetics Research Unit, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Stoneville, MS, United States
Mueller, L. A.;  Boyce Thompson Institute, Ithaca, NY, United States
Mansoor, S.;  National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan
More authors (4 more) Less
Language :
English
Title :
Molecular insight into cotton leaf curl geminivirus disease resistance in cultivated cotton (Gossypium hirsutum)
Publication date :
2019
Journal title :
Plant Biotechnology Journal
ISSN :
1467-7644
eISSN :
1467-7652
Publisher :
Blackwell Publishing Ltd
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
USDA - United States Department of Agriculture [US-DC] [US-DC]
Available on ORBi :
since 06 February 2020

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