CRISPR technology to combat plant RNA viruses: A theoretical model for Potato virus Y (PVY)resistance

[en] RNA viruses are the most diverse phytopathogens which cause severe epidemics in important agricultural crops and threaten the global food security. Being obligatory intracellular pathogens, these viruses have developed fine-tuned evading mechanisms and are non-responsive to most of the prophylactic treatments. Additionally, their sprint ability to overcome host defense demands a broad-spectrum and durable mechanism of resistance. In context of CRISPR-Cas discoveries, some variants of Cas effectors have been characterized as programmable RNA-guided RNases in the microbial genomes and could be reprogramed in mammalian and plant cells with guided RNase activity. Recently, the RNA variants of CRISPR-Cas systems have been successfully employed in plants to engineer resistance against RNA viruses. Some variants of CRISPR-Cas9 have been tamed either for directly targeting plant RNA viruses’ genome or through targeting the host genes/factors assisting in viral proliferation. The new frontiers in CRISPR-Cas discoveries, and more importantly shifting towards RNA targeting will pyramid the opportunities in plant virus research. The current review highlights the probable implications of CRISPR-Cas system to confer the pathogen-derived or host-mediated resistance against phytopathogenic RNA viruses. Furthermore, a multiplexed CRISPR-Cas13a methodology is proposed here to combat Potato virus Y (PVY); a globally diverse phytopathogen infecting multiple crops. © 2019 Elsevier Ltd

Disciplines :

Genetics & genetic processes
Biotechnology
Microbiology
Agriculture & agronomy

Author, co-author :

Hameed, A.; Akhuwat Faisalabad Institute of Research Science and Technology, Faisalabad, Pakistan, Department of Bioinformatics & Biotechnology, Government College University, Allama Iqbal Road, Faisalabad, Pakistan

Syed-Shan-e-Ali, Zaïdi ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Ingénierie des productions végétales et valorisation

Sattar, M. N.; Department of Biotechnology, College of Agriculture and Food Science, King Faisal University, Box 400, Al-Ahsa3192, Saudi Arabia

Iqbal, Z.; Department of Plant Pathology, University of Florida, Gainesville, FL 32611, United States

Tahir, M. N.; Department of Plant Pathology, Bahauddin Zakariya University, Multan, Pakistan

Language :

English

Title :

CRISPR technology to combat plant RNA viruses: A theoretical model for Potato virus Y (PVY)resistance

Publication date :

2019

Journal title :

Microbial Pathogenesis

ISSN :

0882-4010

eISSN :

1096-1208

Publisher :

Academic Press

Volume :

133

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 06 February 2020

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