Slow Freezing Versus Vitrification of Mouse Ovaries: from Ex Vivo Analyses to Successful Pregnancies after Auto-Transplantation

Terren, Carmen; Fransolet, Maïté; Ancion, Marie; Nisolle, Michelle; Munaut, Carine

doi:10.1038/s41598-019-56182-8

Article (Scientific journals)

Slow Freezing Versus Vitrification of Mouse Ovaries: from Ex Vivo Analyses to Successful Pregnancies after Auto-Transplantation

Terren, Carmen; Fransolet, Maïté; Ancion, Marie et al.

2019 • In Scientific Reports, 9 (1), p. 19668

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https://hdl.handle.net/2268/244549

DOI
10.1038/s41598-019-56182-8

PubMed
31873164

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Keywords :

Cryopreservation; Ovary; Slow freezing; Vitrification; Auto-transplantation; Fertility restoration

Abstract :

[en] Slow freezing (SF) is the reference method for ovarian tissue cryopreservation. Vitrification (VT) constitutes an alternative but controversial method. This study compares SF and VT (open [VTo] and closed [VTc] systems) in terms of freezing damage and fertility restoration ability. In vitro analyses of C57Bl/6 SF or VTo-ovaries, immediately after thawing/warming or after culture (cult), revealed that event though follicular density was similar between all groups, nuclear density was decreased in VTo-ovaries compared to CT-ovaries (CT = 0.50 +/- 0.012, SF = 0.41 +/- 0.03 and VTo = 0.29 +/- 0.044, p < 0.01). Apoptosis was higher in VTo-cult ovaries compared to SF-cult ovaries (p < 0.001) whereas follicular Bmp15 and Amh gene expression levels were decreased in the ovaries after culture, mostly after VTo (p < 0.001). Natural mating after auto-transplantation of SF, VTo and VTc-ovaries revealed that most mice recovered their oestrous cycle. Fertility was only restored with SF and VTo ovaries (SF: 68%; VTo: 63%; VTc: 0%; p < 0.001). Mice auto-transplanted with SF and VTo-ovaries achieved the highest number of pregnancies. In conclusion, in vitro, no differences between SF and VTo were evident immediately after thawing/warming but VTo ovaries displayed alterations in apoptosis and follicular specific proteins after culture. In vivo, SF and VTo ovary auto-transplantation fully restored fertility whereas with VTc-ovary auto-transplantation no pregnancies were achieved.

Disciplines :

Reproductive medicine (gynecology, andrology, obstetrics)

Author, co-author :

Terren, Carmen ; Université de Liège - ULiège > Cancer-Tumours and development biology

Fransolet, Maïté ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement

Ancion, Marie ; Université de Liège - ULiège > Cancer-Experimental Pathology

Nisolle, Michelle ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique

Munaut, Carine ; Université de Liège - ULiège > Cancer-Tumours and development biology

Language :

English

Title :

Slow Freezing Versus Vitrification of Mouse Ovaries: from Ex Vivo Analyses to Successful Pregnancies after Auto-Transplantation

Publication date :

23 December 2019

Journal title :

Scientific Reports

eISSN :

2045-2322

Publisher :

Nature Publishing Group, London, United Kingdom

Volume :

Issue :

Pages :

19668

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://www.ncbi.nlm.nih.gov/pubmed/31873164

Commentary :

2019/12/25

Available on ORBi :

since 06 February 2020

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