Article (Scientific journals)
Characterization of New Fengycin Cyclic Lipopeptide Variants Produced by Bacillus amyloliquefaciens (ET) Originating from a Salt Lake of Eastern Algeria.
Ait Kaki, Asma; Smargiasso, Nicolas; Ongena, Marc et al.
2020In Current Microbiology
Peer Reviewed verified by ORBi
 

Files


Full Text
Characterization of New Fengycin Cyclic Lipopeptide Variants Produced by Bacillus Amyloliquefaciens.pdf
Publisher postprint (42.4 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] Fengycin antibiotic displays a strong antifungal activity and inhibits the growth of a wide range of plant pathogens especially filamentous fungi. The main objective of the present study is to characterize fengycin variants produced by B. amyloliquefaciens strain (ET). LC-MS analysis of fengycin extracts has shown several molecular ion peaks corresponding to conventional fengycin homologues (MH + : m/z 1463.9; 1491.9; 1506) and some new ones (MH + : m/z 1433; 1447; 1461; and 1477). Further characterization of these precursor ions was carried out by LC-MS.MS analysis. Reporter fragment ions were observed (named A and B), they correspond to the cleavage of Orn2-Tyr3 (A), Glu1-Orn2 (B), and used for identifying fengycin variants. The reporter fragment couple ions [A/B] at [m/z 966.5/1080.5] and [m/z 994.4 /1108.5] represent fengycin A and B, respectively. The diagnostic ions at ([m/z 980/1094]) may correspond to fengycin C3, D, S or B2. Interestingly, unknown diagnostic product ions at [m/z 951/1065] and [m/z 979/1093] were detected for the first time in this study which prove that they correspond to new fengycin variants, named fengycin X and fengycin Y, respectively. The fengycin X results from a substitution of the glutamine amino acid (Q), at position 8 of the fengycin A peptide part, by an isoleucine (I) or a leucine (L) residue. This mutation should be the same in fengycin Y but compared to fengycin B.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Ait Kaki, Asma 
Smargiasso, Nicolas 
Ongena, Marc ;  Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies
Kara Ali, Mounira
Moula, Nassim  ;  Université de Liège - ULiège > Dpt. de gestion vétérinaire des Ressources Animales (DRA) > Dpt. de gestion vétérinaire des Ressources Animales (DRA)
De Pauw, Edwin  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Kacem Chaouche, Noreddine
Language :
English
Title :
Characterization of New Fengycin Cyclic Lipopeptide Variants Produced by Bacillus amyloliquefaciens (ET) Originating from a Salt Lake of Eastern Algeria.
Publication date :
2020
Journal title :
Current Microbiology
ISSN :
0343-8651
eISSN :
1432-0991
Publisher :
Springer, Germany
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 06 February 2020

Statistics


Number of views
99 (8 by ULiège)
Number of downloads
5 (5 by ULiège)

Scopus citations®
 
14
Scopus citations®
without self-citations
13
OpenCitations
 
12

Bibliography


Similar publications



Contact ORBi