Article (Scientific journals)
The Enterococcus faecalis virulence factor ElrA interacts with the human Four-And-A-Half LIM Domains Protein
Jamet, Alexandre; Dervyn, Rozenn; Lapaque, Nicolas et al.
2017In Scientific Reports, 7 (1)
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Abstract :
[en] The commensal bacterium Enterococcus faecalis is a common cause of nosocomial infections worldwide. The increasing prevalence of multi-Antibiotic resistant E. faecalis strains reinforces this public health concern. Despite numerous studies highlighting several pathology-related genetic traits, the molecular mechanisms of E. faecalis virulence remain poorly understood. In this work, we studied 23 bacterial proteins that could be considered as virulence factors or involved in the Enterococcus interaction with the host. We systematically tested their interactions with human proteins using the Human ORFeome library, a set of 12,212 human ORFs, in yeast. Among the thousands of tested interactions, one involving the E. faecalis virulence factor ElrA and the human protein FHL2 was evidenced by yeast two-hybrid and biochemically confirmed. Further molecular characterizations allowed defining an FHL2-interacting domain (FID) of ElrA. Deletion of the FID led to an attenuated in vivo phenotype of the mutated strain clearly indicating that this interaction is likely to contribute to the multifactorial virulence of this opportunistic pathogen. Altogether, our results show that FHL2 is the first host cellular protein directly targeted by an E. faecalis virulence factor and that this interaction is involved in Enterococcus pathogenicity. © 2017 The Author(s).
Disciplines :
Microbiology
Author, co-author :
Jamet, Alexandre;  Micalis Institute, INRA AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France
Dervyn, Rozenn;  Micalis Institute, INRA AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France
Lapaque, Nicolas;  Micalis Institute, INRA AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France
Bugli, Francesca;  Institute of Microbiology, University Cattolica Del Sacro Cuore, Rome, Italy
Perez-Cortez, Naima G.;  Micalis Institute, INRA AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France, INRA, Unité d'Immuno-Allergie Alimentaire France, iBiTecS/SPI, Gif-sur-Yvette, France
Blottière, Hervé M.;  Micalis Institute, INRA AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France
Twizere, Jean-Claude  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Sanguinetti, Maurizio;  Institute of Microbiology, University Cattolica Del Sacro Cuore, Rome, Italy
Posteraro, Brunella;  Institute of Public Health, Section of Hygiene, Universita Cattolica Del Sacro Cuore, Rome, Italy
Serror, Pascale;  Micalis Institute, INRA AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France
Maguin, Emmanuelle;  Micalis Institute, INRA AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France
Language :
English
Title :
The Enterococcus faecalis virulence factor ElrA interacts with the human Four-And-A-Half LIM Domains Protein
Publication date :
2017
Journal title :
Scientific Reports
eISSN :
2045-2322
Publisher :
Nature Publishing Group
Volume :
7
Issue :
1
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 20 January 2020

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