The Enterococcus faecalis virulence factor ElrA interacts with the human Four-And-A-Half LIM Domains Protein

[en] The commensal bacterium Enterococcus faecalis is a common cause of nosocomial infections worldwide. The increasing prevalence of multi-Antibiotic resistant E. faecalis strains reinforces this public health concern. Despite numerous studies highlighting several pathology-related genetic traits, the molecular mechanisms of E. faecalis virulence remain poorly understood. In this work, we studied 23 bacterial proteins that could be considered as virulence factors or involved in the Enterococcus interaction with the host. We systematically tested their interactions with human proteins using the Human ORFeome library, a set of 12,212 human ORFs, in yeast. Among the thousands of tested interactions, one involving the E. faecalis virulence factor ElrA and the human protein FHL2 was evidenced by yeast two-hybrid and biochemically confirmed. Further molecular characterizations allowed defining an FHL2-interacting domain (FID) of ElrA. Deletion of the FID led to an attenuated in vivo phenotype of the mutated strain clearly indicating that this interaction is likely to contribute to the multifactorial virulence of this opportunistic pathogen. Altogether, our results show that FHL2 is the first host cellular protein directly targeted by an E. faecalis virulence factor and that this interaction is involved in Enterococcus pathogenicity. © 2017 The Author(s).

Disciplines :

Microbiology

Author, co-author :

Jamet, Alexandre; Micalis Institute, INRA AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France

Dervyn, Rozenn; Micalis Institute, INRA AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France

Lapaque, Nicolas; Micalis Institute, INRA AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France

Bugli, Francesca; Institute of Microbiology, University Cattolica Del Sacro Cuore, Rome, Italy

Perez-Cortez, Naima G.; Micalis Institute, INRA AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France, INRA, Unité d'Immuno-Allergie Alimentaire France, iBiTecS/SPI, Gif-sur-Yvette, France

Blottière, Hervé M.; Micalis Institute, INRA AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France

Twizere, Jean-Claude ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies

Sanguinetti, Maurizio; Institute of Microbiology, University Cattolica Del Sacro Cuore, Rome, Italy

Posteraro, Brunella; Institute of Public Health, Section of Hygiene, Universita Cattolica Del Sacro Cuore, Rome, Italy

Serror, Pascale; Micalis Institute, INRA AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France

Maguin, Emmanuelle; Micalis Institute, INRA AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France

Language :

English

Title :

The Enterococcus faecalis virulence factor ElrA interacts with the human Four-And-A-Half LIM Domains Protein

Publication date :

2017

Journal title :

Scientific Reports

eISSN :

2045-2322

Publisher :

Nature Publishing Group

Volume :

Issue :

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 20 January 2020

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