Effects of sex and fasting/refeeding on hepatic AMPK signaling in chickens (Gallus gallus).

[en] The alpha-1 isoform of chicken AMPK situates on the Z-chromosome, in contrast, the other isoforms in birds and the mammalian AMPKalpha1 are located on the autosomes. The present study aimed to investigate the role of hepatic AMPK signaling in adaptation to nutritional status and the potential sex-specific response in chickens. Hepatic genes and proteins were compared between the two sexes immediately after hatching. From 20d of age, chicks from each sex received feed treatments: Control was fed ad libitum; Fasted was starved for 24h; Refed was fed for 4h after a 24h fasting. As a result, hepatic AMPKalpha1 mRNA level in males was significantly higher at both ages compared to females, due to the presence of Z-chromosomes. However, this did not make this kinase "male-bias" as it was eventually compensated at a translational level, which was not reported in previous studies. The protein levels and activation of AMPKalpha were even lower in newly-hatched male compared to female chicks, accompanied with a higher FAS and SREBP-1 gene expressions. Accordingly, hepatic G6PC2 mRNA levels in males were significantly lower associated with lower plasma glucose levels after hatching. Fasting activated hepatic AMPK, which in turn inhibited gene expression of GS, FAS and SREBP-1, and stimulated the downstream G6PC2 in both sexes. These changes recovered after refeeding. In conclusion, AMPK plays a role in adaptation to nutritional environment for both sexes. The Z-linked AMPK did not exert a sex-specific signaling, due to a "translational compensation" of AMPKalpha1.

Disciplines :

Agriculture & agronomy

Author, co-author :

Wang, Yufeng

Buyse, Johan

Courousse, Nathalie

Tesseraud, Sophie

Metayer-Coustard, Sonia

Berri, Cecile

Schallier, Seline

Everaert, Nadia ; Université de Liège - ULiège > Département GxABT > Ingénierie des productions animales et nutrition

Collin, Anne

Language :

English

Title :

Effects of sex and fasting/refeeding on hepatic AMPK signaling in chickens (Gallus gallus).

Publication date :

2020

Journal title :

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

ISSN :

1095-6433

eISSN :

1531-4332

Volume :

240

Pages :

110606

Peer reviewed :

Peer reviewed

Commentary :

Available on ORBi :

since 17 January 2020

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