[en] Phytochemical investigation of the ethanol extract of the stem bark of Hypericum lanceolatum Lam. (Guttifereae)
afforded eight known compounds including 2,2ʹ,5,6ʹ-tetrahydroxybenzophenone (1), 5-hydroxy-3-methoxyxanthone
(2), 3-hydroxy-5-methoxyxanthone (3), betulinic acid (4), hydroquinone (5) 6,7-dihydroxy-1,3-dimethoxyxanthone
(6), calophyllumin A (7), and 1,3,5,6-tetrahydroxy-4-prenylxanthone (8). Compound 1 was submitted to acetylation
reaction to give 5-acetoxy-2,2ʹ,6ʹ-trihydroxybenzophenone (9), a new hemisynthetic derivative. Compounds 5 and
8 were isolated for the first time from this plant. The structures were established by extensive analysis of their mass
spectrometry and nuclear magnetic resonance (NMR) spectroscopic data and comparison with those from the literature.
The isolated compounds (1, 2, 4, 5, and 8) and the derivative of benzophenone (9) were tested for their antipromastigote
and cytotoxic activities against visceral leishmaniasis parasite Leishmania donovani and macrophage RAW 264.7 cell
line, respectively. Compound 9 was the most active with an IC50 value of 6.1 μg/ml, while compounds 1, 2, 4, and 8
were moderately active with IC50 values ranging from 11.4 to 34.8 μg/ml against L. donovani and were not cytotoxic
except compound 5 that was very toxic and not active. The findings of the present study suggested that compounds 1,
2, 4, and 8 could be considered as a potential source of therapeutic medicine for the treatment of leishmaniasis.
Research Center/Unit :
CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Kowa, Théodora; Institute of Medical Research and Medicinal PlantsStudies, Yaounde
Tchokouaha, Lauve; Institute of Medical Research and Medicinal PlantsStudies, Yaounde
Nyemb, Jean-Noël; University of Ngaoundere
Wabo, Hippolyte; University of Dschang
Tchinda, Alembert; Institute of Medical Research and Medicinal PlantsStudies, Yaounde
Appiah-Opong, Regina; University of Ghana
Agbor, Gabriel
Tane, Pierre
Frederich, Michel ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Language :
English
Title :
Antipromastigote and cytotoxic activities of some chemical constituents of Hypericum lanceolatum Lam.
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