Poster (Scientific congresses and symposiums)
Assessment of the feasibility to develop a fast and easy reproducible 3D bronchial model growing at the air-liquid interface: Which critical culture parameters must be controlled?
Lechanteur, Anna; Evrard, Brigitte; Piel, Géraldine
20192nd CIRM Day
 

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Abstract :
[en] The discovery and evaluation of new active drugs will be easier thanks to the development of 3D-epithelial model mimicking biological barriers (1). These models closer to in vivo conditions should help to speed up the drug development process. Regarding lung administration, despite many studies exploring the development of 2D-model using Calu-3 cells, there are still multiple disparities about experimental methods and a standardization is needed. We investigated the impact of different culture parameters (time, cell densities,…) on the integrity and the morphology of a 2D model (2). Using permeability studies, electron microscopy and immunohistochemistry, we propose an easy and reproducible 2D-model with Calu-3 cells fully differentiated after 14 days of growing at the air-liquid interface. Moreover, based on these results, we went further with a 3D-bronchial model which consists of a collagen matrix, fibroblasts laid under the epithelial layer. We aim to assess the feasibility to develop a 3D cell-based model of the human airway growing at the air-liquid interface. Many parameters which have to be tightly controlled to develop an extracellular matrix equivalent without any shrinking of the gel encompassing alive fibroblasts as well as over epithelial cells have been identified. Overall, this in vitro model is a differentiated pseudo-stratified bronchial mucosa with mucus expression which is the basis for further optimization allowing the screening of pulmonary drugs in terms of permeability, cytotoxicity or particle-mucus interactions. (1) A. Lechanteur, et al. ADDR. 124 (2018) 50–63. (2) A. Lechanteur, et al. EJPB. 144 (2019) 2–10.
Research center :
CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Lechanteur, Anna  ;  Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique
Evrard, Brigitte  ;  Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique
Piel, Géraldine ;  Université de Liège - ULiège > Département de pharmacie > Développement de nanomédicaments
Language :
English
Title :
Assessment of the feasibility to develop a fast and easy reproducible 3D bronchial model growing at the air-liquid interface: Which critical culture parameters must be controlled?
Publication date :
11 December 2019
Event name :
2nd CIRM Day
Event organizer :
CIRM
Event date :
11 décembre 2019
Available on ORBi :
since 18 December 2019

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