Article (Scientific journals)
Rewiring of Lipid Metabolism and Storage in Ovarian Cancer Cells after Anti-VEGF Therapy
Curtarello, Matteo; Tognon, Martina; Venturoli, Carolina et al.
2019In Cells
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Keywords :
Angiogenèse; Resistance; Métabolisme
Abstract :
[en] Anti-angiogenic therapy triggers metabolic alterations in experimental and human tumors, the best characterized being exacerbated glycolysis and lactate production. By using both Liquid Chromatography-Mass Spectrometry (LC-MS) and Nuclear Magnetic Resonance (NMR) analysis, we found that treatment of ovarian cancer xenografts with the anti-Vascular Endothelial Growth Factor (VEGF) neutralizing antibody bevacizumab caused marked alterations of the tumor lipidomic profile, including increased levels of triacylglycerols and reduced saturation of lipid chains. Moreover, transcriptome analysis uncovered up-regulation of pathways involved in lipid metabolism. These alterations were accompanied by increased accumulation of lipid droplets in tumors. This phenomenon was reproduced under hypoxic conditions in vitro, where it mainly depended from uptake of exogenous lipids and was counteracted by treatment with the Liver X Receptor (LXR)-agonist GW3965, which inhibited cancer cell viability selectively under reduced serum conditions. This multi-level analysis indicates alterations of lipid metabolism following anti-VEGF therapy in ovarian cancer xenografts and suggests that LXR-agonists might empower anti-tumor e ects of bevacizumab.
Disciplines :
Oncology
Author, co-author :
Curtarello, Matteo
Tognon, Martina
Venturoli, Carolina
Silic-Benussi, Micol
Grassi, Angela
Verza, Martina
Minuzzo, Sonia
Pinazza, Marica
Brillo, Valentina
Tosi, Giovanni
Ferrazza, Ruggero
Guella, Graziano
Iorio, Egidio
Godfroid, Adrien ;  Université de Liège - ULiège > Cancer-Tumours and development biology
Sounni, Nor Eddine  ;  Université de Liège - ULiège > Cancer-Tumours and development biology
Amadori, Alberto
Stefano
More authors (7 more) Less
Language :
English
Title :
Rewiring of Lipid Metabolism and Storage in Ovarian Cancer Cells after Anti-VEGF Therapy
Publication date :
2019
Journal title :
Cells
eISSN :
2073-4409
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI), Basel, Switzerland
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 18 December 2019

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