Article (Scientific journals)
Cowpea aphid–plant interactions: endosymbionts and related salivary protein patterns
Bayendi Loudit, Sandrine; Bauwens, Julien; Francis, Frédéric
2018In Entomologia Experimentalis et Applicata, 166 (6), p. 460-473
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Keywords :
Amaranthaceae; Amaranthus hybridus; Aphididae; Aphis craccivora; Hemiptera; Serratia sp.; Benin [West Africa]; Gabon; Amaranthus; Aphidini; Bacteria (microorganisms); Buchnera aphidicola; Candidatus Serratia symbiotica; Enterobacteriaceae; Fabaceae; Hexapoda; Rickettsia sp.; Rickettsiaceae; Vigna unguiculata
Abstract :
[en] The specificity of plant use by aphids is related to symbiont diversity of some aphid models. Aphis craccivora Koch (Hemiptera: Aphididae, Aphidini) is a well-known aphid that feeds on species of Fabaceae, but has also been recorded recently on Amaranthus species (Amaranthaceae) in Gabon (Africa). Aphis craccivora strains used in this study were originally collected from crop Vigna unguiculata L. Walp. (Fabaceae) from Togba in Benin (Africa) and Amaranthus hybridus L. from Libreville in Gabon, for a comparative study of symbionts. Saliva composition, potentially including bacterial proteins, also contributes to the phytotoxic effect of aphid attacks. Both, endosymbiont bacteria and saliva protein diversity should be targeted to investigate the feeding behavior of aphids and to explain plant–aphid interactions. Bacteria-targeted PCR was conducted on six symbionts in A. craccivora. The obligate symbiont Buchnera aphidicola Munson et al. (Enterobacteriaceae) was identified in all aphids collected. In comparison, the facultative symbiont Serratia symbiotica Moran et al. (Enterobacteriaceae) was only found in A. craccivora from Gabon, whereas Rickettsia sp. (Rickettsiaceae) was only found in aphids from Benin. Using nano-LC-MS/MS (liquid chromatography-tandem mass spectrometry), some proteins were only found in solid or soluble saliva, whereas others originated from S. symbiotica. Two of the identified proteins are involved in plant–pathogen interactions: calmodulin and elongation factor Tu. This information on endosymbionts and related salivary proteomes from A. craccivora in Gabon helps improve our understanding of aphid–plant interactions. © 2018 The Netherlands Entomological Society
Disciplines :
Entomology & pest control
Author, co-author :
Bayendi Loudit, Sandrine ;  Entomologie fonctionnelle et évolutive, Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, Gembloux, 5030, Belgium, Institut de Recherches Agronomiques et Forestières, BP 13 260, Libreville, Gabon
Bauwens, Julien ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Gestion durable des bio-agresseurs
Francis, Frédéric  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Gestion durable des bio-agresseurs
Language :
English
Title :
Cowpea aphid–plant interactions: endosymbionts and related salivary protein patterns
Publication date :
2018
Journal title :
Entomologia Experimentalis et Applicata
ISSN :
0013-8703
Publisher :
Blackwell Publishing Ltd
Volume :
166
Issue :
6
Pages :
460-473
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 13 December 2019

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