Biochemical and structural insights into the interaction of the essential domain of FtsN with PBP1b

The mutation W83L within eFtsN reduced binding of FtsN to PBP1b and the activation of PBP1b
Evidence for the interaction between PBP1b and eFtsN was shown using different techniques including FP binding assay, activity assay, cross-linking and x-ray crystallography.
eFtsN is sufficient for the specific activation of PBP1b.

These results demonstrate that PBP1b is a target of eFtsN and suggest that the direct interaction between eFtsN and PBP1b is the trigger of septal PG synthesis and cell constriction in E. coli.