Article (Scientific journals)
Primary Cilia Signaling Promotes Axonal Tract Development and Is Disrupted in Joubert Syndrome-Related Disorders Models
Guo, Jiami; Otis, James M.; Suciu, Sarah K. et al.
2019In Developmental Cell, 51, p. 759-774
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Keywords :
Inpp5e; 5-phosphatase; phosphoinositides; Neuron; primary cilium; axon; Joubert syndrome
Abstract :
[en] Appropriate axonal growth and connectivity are essential for functional wiring of the brain. Joubert syndrome-related disorders (JSRD), a group of ciliopathies in which mutations disrupt primary cilia function, are characterized by axonal tract malformations. However, little is known about how cilia-driven signaling regulates axonal growth and connectivity. We demonstrate that the deletion of related JSRD genes, Arl13b and Inpp5e, in projection neurons leads to de-fasciculated and misoriented axonal tracts. Arl13b deletion disrupts the function of its downstream effector, Inpp5e, and deregulates ciliary-PI3K/AKT signaling. Chemogenetic activation of ciliary GPCR signaling and cilia-specific optogenetic modulation of downstream second messenger cascades (PI3K, AKT, and AC3) commonly regulated by ciliary signaling receptors induce rapid changes in axonal dynamics. Further, Arl13b deletion leads to changes in transcriptional landscape associated with dysregulated PI3K/AKT signaling. These data suggest that ciliary signaling acts to modulate axonal connectivity and that impaired primary cilia signaling underlies axonal tract defects in JSRD.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Guo, Jiami
Otis, James M.
Suciu, Sarah K.
Catalano, Christy
Xing, Lei
Constable, Sandi
Wachten, Dagmar
Gupton, Stéphanie
Lee, Janice
Lee, Amélia
Blackley, Katherine H.
Ptacek, Travis
Simon, Jérémy M.
Schurmans, Stéphane  ;  Université de Liège - ULiège > Département des sciences fonctionnelles (DSF) > Biochimie métabolique vétérinaire
Stuber, Garret D.
Caspary, Tamara
Anton, E. S.
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Title :
Primary Cilia Signaling Promotes Axonal Tract Development and Is Disrupted in Joubert Syndrome-Related Disorders Models
Publication date :
Journal title :
Developmental Cell
Publisher :
Cell Press, United States
Volume :
Pages :
Peer reviewed :
Peer Reviewed verified by ORBi
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since 13 November 2019


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