Intertwined metal homeostasis, oxidative and biotic stress responses in the Arabidopsis frd3 mutant

[en] FRD3 (FERRIC REDUCTASE DEFECTIVE 3) plays a major role in iron (Fe) and zinc (Zn) homeostasis in Arabidopsis. It transports citrate, which enables metal distribution in the plant. A frd3 mutant is dwarf and chlorotic and displays constitutive Fe deficiency response and strongly altered metal distribution in tissues. Here, we have examined the interaction between Fe and Zn homeostasis in a frd3 mutant exposed to varying Zn supply. Detailed phenotyping using transcriptomic, ionomic, histochemical and spectroscopic approaches revealed the full complexity of the frd3 mutant phenotype, which resulted from altered transition metal homeostasis, manganese toxicity, oxidative and biotic stress responses. The cell wall played a key role in these processes, as a site for Fe and hydrogen peroxide accumulation, and displayed modified structure in the mutant. Finally, we showed that Zn excess interfered with these mechanisms and partially restored root growth of the mutant, without reverting the Fe deficiency response. In conclusion, the frd3 mutant molecular phenotype is more complex than previously described, and illustrates how the response to metal imbalance depends on multiple signaling pathways.

Disciplines :

Phytobiology (plant sciences, forestry, mycology...)

Author, co-author :

Scheepers, Maxime ; Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale

Spielmann, Julien ; Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale

Boulanger, Madeleine ; Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes

Carnol, Monique ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Ecologie végétale et microbienne

Bosman, Bernard ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Ecologie végétale et microbienne

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique

Goormaghtigh, Eric; 5- Structure and Function of Biological membranes, Center for Structural Biology and Bioinformatics, Université Libre de Bruxelles, 1050 Brussels, Belgium

Motte, Patrick ; Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale

Hanikenne, Marc ; Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale

Language :

English

Title :

Intertwined metal homeostasis, oxidative and biotic stress responses in the Arabidopsis frd3 mutant

Publication date :

2020

Journal title :

Plant Journal

ISSN :

0960-7412

eISSN :

1365-313X

Publisher :

Wiley

Volume :

102

Pages :

34-52

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 08 November 2019

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