[en] Resident tissue macrophages (RTM) can fulfill various tasks during development, homeostasis, inflammation and repair. In the lung, non-alveolar RTM, called interstitial macrophages (IM), importantly contribute to tissue homeostasis but remain little characterized. Here we show, using single-cell RNA-sequencing (scRNA-seq), two phenotypically distinct subpopulations of long-lived monocyte-derived IM, i.e. CD206(+) and CD206(-)IM, as well as a discrete population of extravasating CD64(+)CD16.2(+) monocytes. CD206(+) IM are peribronchial self-maintaining RTM that constitutively produce high levels of chemokines and immunosuppressive cytokines. Conversely, CD206(-)IM preferentially populate the alveolar interstitium and exhibit features of antigen-presenting cells. In addition, our data support that CD64(+)CD16.2(+) monocytes arise from intravascular Ly-6C(lo) patrolling monocytes that enter the tissue at steady-state to become putative precursors of CD206(-)IM. This study expands our knowledge about the complexity of lung IM and reveals an ontogenic pathway for one IM subset, an important step for elaborating future macrophage-targeted therapies.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Schyns, Joey ; Université de Liège - ULiège > I3-Immunophysiology
Bai, Qiang ; Université de Liège - ULiège > I3-Immunophysiology
Farnir, Frédéric ; Université de Liège - ULiège > Dpt. de gestion vétérinaire des Ressources Animales (DRA) > Biostatistiques et bioinformatique appliquées aux sc. vétér.
Pirottin, Dimitri ; Université de Liège - ULiège > Département des sciences fonctionnelles (DSF) > Département des sciences fonctionnelles (DSF)
Ginhoux, Florent
Boeckxstaens, Guy
Bureau, Fabrice ; Université de Liège - ULiège > Vice-Recteur à la Recherche
Marichal, Thomas ; Université de Liège - ULiège > I3-Immunophysiology
Language :
English
Title :
Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung.
Publication date :
2019
Journal title :
Nature Communications
eISSN :
2041-1723
Publisher :
Nature Publishing Group, United Kingdom
Volume :
10
Issue :
1
Pages :
3964
Peer reviewed :
Peer Reviewed verified by ORBi
European Projects :
H2020 - 801823 - IM-ID - Defining the intrinsic transcriptional programs and the microenvironmental signals tailoring lung Interstitial Macrophage IDentity
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