[en] Honey composition and color depend greatly on the botanical and geographical origin.
Water content, water activity and color of 50 declared acacia samples, collected from three di erent geographical zones of Romania, together with chromatographic determination of sugar spectrum were analyzed. A number of 79 volatile compounds from the classes of: Alcohols, aldehydes, esters, ketones, sulphur compounds, aliphatic hydrocarbons, nitrogen compounds, carboxylic acids, aromatic acids and ethers were identified by solid-phase micro-extraction and gas-chromatography mass spectrometry. The overall volatile profile and sugar spectrum of the investigated honey samples allow the di erentiation of geographical origin for the acacia honey samples subjected to analysis. The statistical models of the chromatic determination, physicochemical parameters and volatile profile was optimal to characterize the honey samples and group them into three geographical origins, even they belong to the same botanical origin.
Disciplines :
Chemistry Agriculture & agronomy Entomology & pest control
SAFE-HONEY (PN-III=P2-890/12.09.2017 + "Increasing the institutional performance through consolidation and development of research directions within the USAMVCN”
Funders :
Program 1 - Development of the National Research and Development System Subprogram 1.2 - Institutional Performance - Projects for Financing the Excellence in CDI, Contract no. 37PFE/06.11.2018.
EU Council. Council Directive 2001/110/EC of 20 December 2001 relating to honey. Off. J. Eur. Commun. 2002, 10, 47-52.
Alvarez-Suarez, J.M.; Tulipani, S.; Romandini, S.; Vidal, S.A.; Battino, M. Methodological Aspects about Determination of Phenolic Compounds and in Vitro Evaluation of Antioxidant Capacity in the Honey: A Review. Curr. Anal. Chem. 2009, 5, 293-302. [CrossRef]
Anklam, E. A review of the analytical methods to determine the geographical and botanical origin of honey. Food Chem. 1998, 63, 549-562. [CrossRef]
Escuredo, O.; Dobre, I.; Fernández-González, M.; Seijo, M.C. Contribution of botanical origin and sugar composition of honeys on the crystallization phenomenon. Food Chem. 2014, 149, 84-90. [CrossRef] [PubMed]
Oroian, M.; Amariei, S.; Leahu, A.; Gut, G. Multi-Element Composition of Honey as a Suitable Tool for Its Authenticity Analysis. Polish J. Food Nutr. Sci. 2015, 65, 93-100. [CrossRef]
Estevinho, L.M.; Feás, X.; Seijas, J.A.; Pilar Vázquez-Tato, M.P. Organic honey from Trás-os-Montes Region (Portugal): Chemical, palinological, microbiological and bioactive compounds characterization. Food Chem. Toxicol. 2012, 50, 258-264. [CrossRef] [PubMed]
Kaškoniene, V.; Venskutonis, P.R. Floral markers in honey of various botanical and geographical origins: A Review. Compr. Rev. Food Sci. Food 2010, 9, 620-634. [CrossRef]
Karabagias, I.K.; Maia, M.; Karabagias, V.K.; Gatzias, I.; Badeka, A.V. Characterization of Eucaliptus, Chestnut and Heather Honeys from Portugal Using Multi-Parameter Analysis and Chemo-Calculus. Foods 2018, 7, 194. [CrossRef] [PubMed]
Manyi-Loh, C.E.; Ndip, R.N.; Clarke, A.M. Volatile Compounds in Honey: A review on Their Involvment in Aroma, Botanical Origin Determination and Potential Biomedical Activities. Int. J. Mol. Sci. 2011, 12, 9514-9532. [CrossRef] [PubMed]
Juan-Borrás, M.; Domenech, E.; Escheriche, I. Correlation between methyl anthranilate level and percentage of pollen in Spanish citrus honey. Int. J. Food Sci. Technol. 2015, 50, 1690-1696. [CrossRef]
Juan-Borrás, M.; Domenech, E.; Hellebrandova, M.; Escheriche, I. Effect of country origin on physicochemical, sugar and volatile composition of acacia, sunflower and tilia honeys. Food Res. Int. 2014, 60, 86-94. [CrossRef]
Kádár, M.; Juan-Borrás, M.; Carot, J.M.; Domenech, E.; Escheriche, I. Volatile fraction composition and physicochemical parameters as tools for the differentiation of lemon blossom honeys and orange blossom honey. J. Sci. Food Agric. 2011, 91, 2768-2776. [CrossRef] [PubMed]
Persano-Oddo, L.; Piro, R. Main European unifloral honeys: Descriptive sheets. Apidologie 2004, 35, 38-81. [CrossRef]
Mărghitaş, L.A.; Dezmirean, D.; Moise, A.; Bobiş, O.; Laslo, L.; Bogdanov, S. Physico-chemical and bioactive properties of different floral origin honeys from Romania. Food Chem. 2009, 112, 863-867. [CrossRef]
Karabagias, I.K.; Karabournioti, S. Discrimination of Clover and Citrus Honeys from Egypt According to Floral Type Using Easily Assessable Physicochemical Parameters and Discriminant Analysis: An External Validation of the Chemometric Approach. Foods 2018, 7, 70. [CrossRef] [PubMed]
Bonta, V.; Mărghitaş, L.A.; Stanciu, O.; Laslo, L.; Dezmirean, D.; Bobiş, O. High performance liquid chromatographic analysis of sugars in Transilvanian honeydew honey. USAMVCN Bull. 2008, 65, 229-232.
Cotte, J.F.; Casabianca, H.; Chardon, S.; Lheritier, J.; Grenier-Loustalot, M.F.G. Chromatographic analysis of sugars applied to the characterization of monofloral honey. Anal. Bioanal. Chem. 2004, 280, 698-705. [CrossRef]
Escheriche, I.; Sobrino-Gregorio, L.; Conchado, A.; Juan-Borrás, M. Volatile profile in the accurate labelling of monofloral honey. The case of lavander and thyme honey. Food Chem. 2017, 226, 61-68. [CrossRef]
Gianelli Barra, M.P.; Ponce-Díaz, M.C.; Venegas-Gallegos, C. Volatile compounds in honey produced in the central valley of Ñuble Province, Chile. Chil. J. Agric. Res. 2010, 70, 75-84. [CrossRef]
Hafida, H.; Fauconnier, M.L.; Rizki, H.; Ouradi, H.; Ennahli, S.; Hassaini, L. Determination of physico-chemical properties and composition in volatile constituents by Solid Phase Micro-Extraction of honey samples from different botanical and geographical origins in Morocco. J. Apic. Res. 2019, in press.
Kádár, M.; Juan-Borrás, M.; Hellebrandova, M.; Doménech, E.; Escheriche, I. Volatile Fraction Composition of Acacia (Robinia pseudoacacia) Honey from Romania, Spain and Check Republic. Bull. USAMV Agric. 2010, 67, 259-265.
Pérez, R.A.; Sánchez-Brunete, C.; Calvo, R.M.; Tadeo, J.L. Analysis of Volatiles from Spain Honeys by Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry. J. Agric. Food Chem. 2002, 50, 2633-2637. [CrossRef]
Cimpoiu, C.; Hosu, A.; Miclăuş, V.; Puşcaş, A. Determination of floral origin of some Romanian honeys on the basis of physical and biochemical properties. Spectrochim. Acta Part A 2013, 100, 149-154. [CrossRef]
Dobre, I.; Alexe, P.; Escuredo, O.; Seijo, C.M. Palynological evaluation of selected honeys from Romania. Grania 2013, 52, 113-121. [CrossRef]
Dincă, O.R.; Ionete, R.E.; Popescu, R.; Costinel, D.; Radu, G.L. Geographical and botanical origin discrimination of Romanian honey using complex stable isotope data and chemometrics. Food Anal. Meth. 2015, 8, 401-412. [CrossRef]
Stihi, C.; Chelarescu, E.; Duliu, O.G.; Toma, L.G. Characterization of Romanian honey using physico-chemical parameters and the elemental content determined by analytical techniques. Rom. Rep. Phys. 2016, 68, 362-369.
Oroian, M.; Amariei, S.; Roşu, A.; Gutt, G. Classification of unifloral honeys using multivariate analysis. J. Essent. Oil Res. 2015, 27, 533-544. [CrossRef]
Oroian, M.; Ropciuc, S.; Buculei, A. Romanian honey authentication based on phisico-chermical parameters and chemometrics. J. Food Meas. Charact. 2016, 1-7. [CrossRef]
SR 784/1-3:2009. Honey. Part 1: Quality requirements at delivery by producers; Part 2: Honey quality requirements; Part 3: Analysis methods. Available online: https://magazin.asro.ro/standarde/can/1/S84 (accessed on 25 August 2019).
Bogdanov, S.; Martin, P.; Lüllmann, C. Harmonised methods of the European Honey Commission. Apidologie 1997, 1-59. Available online: http://www.ihc-platform.net/ihcmethods2009.pdf (accessed on 25 August 2019).
Tanoh, E.A.; Nea, F.; Kemene, T.K.; Genva, M.; Saive, M.; Tonzibo, F.Z.; Fauconnier, M.L. Antioxidant and Lipoxygenase Inhibitory Activities of Essential Oils from Endemic Plants of Côte d'Ivoire: Zanthoxylum mezoneurispinosum Ake Assi and Zanthoxylum psammophilum Ake Assi. Molecules 2019, 24, 2445. [CrossRef]
Czipa, N.; Phillips, C.J.C.; Kovács, B. Composition of acacia honeys following processing, storage and adulteration. J. Food Sci. Technol. 2019, 56, 1245-1255. [CrossRef]
Lazarevic, K.B.; Andric, F.; Trifkovic, J.; Tesik, Z.; Milojkovic-Opsenica, D. Characterization of Serbian unifloral honeys according to their physicochemical parameters. Food Chem. 2012, 132, 2060-2064. [CrossRef]
Pridal, A.; Vorlova, L. Honey and its physical parameters. Czech J. Anim. Sci. 2002, 47, 439-444.
Uršulin-Trstenjak, N.; Puntarić, D.; Levanić, D.; Gvozdić, V.; Pavlek, Ž.; Puntarić, A.; Puntarić, E.; Puntarić, I.; Vidosavljević, D.; Lasić, D.; et al. Pollen, physicochemical and mineral analysis of Croation Acacia honey samples: Applicability for identificaton of botanical and geographical origin. J. Food Qual. 2017, 2017, 8538693. [CrossRef]
Kasperová, J.; Nagy, J.; Popelka, P.; Dičáková, Z.; Nagyová, A.; Malá, P. Physico-chemical indicators and identification of selected Slovak honeys based on color measurement. Acta Vet. Brno 2012, 81, 057-062. [CrossRef]
Ropciuc, S.; Oroian, M.; Olariu, V. Impact of adulteration with glucose, fructose and hydrolysed inulin syrup on honey physical-chemical properties. Food Environ. Saf. 2017, 16, 54-60.
Bertoncelj, J.; Doberšek, U.; Jamnik, M.; Golob, T. Evaluation of the phenolic content, antioxidant activity and color of Slovenian honey. Food Chem. 2007, 105, 822-828. [CrossRef]
Escuredo, O.; Rodriguez-Flores, M.S.; Rojo-Martinez, S.; Seijo, C.M. Contribution to the chromatic characterization of unifloral honeys from Galicia (NW Spain). Foods 2019, 8, 233. [CrossRef]
Marc, L.; Mărghitaş, L.A.; Bobiş, O.; Bonta, V.; Mihai, C. Preliminary study on the authenticity of Acacia, Multifloral and Linden honey from Cluj County. Bull. USAMV Anim. Sci. Biotechnol. 2012, 69, 129-135.
Golob, T.; Plestenjak, A. Quality of Slovene honey. Food Technol. Biotechnol. 1999, 37, 195-201.
Sanz, M.L.; Gonzalez, M.; de Lorenzo, C.; Sanz, L.; Martinez-Castro, I. Carbohydrate composition and physic chemical properties of artisanal honeys from Madrid (Spain): Occurrence of Echium sp honey. J. Sci. Food Agric. 2004, 84, 1577-1584. [CrossRef]
Bobiş, O.; Mărghitaş, L.; Bonta, V.; Dezmirean, D.; Cocan, O. Determination of sugars in Romanian honeys of different botanical origin, using HPLC. Bull. USAMV 2006, 62, 143-148.
Bonta, V.; Mărghitaş, L.; Bobiş, O.; Dezmirean, D. HPLC sugar profile in quality and authenticity determination of Romanian honey. In Apiculture-From Science to Agribusiness and Apitherapy; Maronia Publishing House: Cluj-Napoca, Romania, 2007; pp. 59-64.
Mărghitaş, L.A.; Dezmirean, D.; Pocol, C.; Ilea, M.; Bobiş, O.; Gergen, I. The Development of a Biochemical Profile of Acacia Honey by identifying Biochemical Determinants of its Quality. Not. Bot. Horti Agrobot. 2010, 38, 84-90. [CrossRef]
Mădaş, N.; Mărghitaş, L.A.; Dezmirean, D.; Bonta, V.; Bobiş, O. Botanical origin authentication of Black locust (Robinia pseudoacacia) honey, by means of sugar spectrum determnination. Agricultura 2012, 3, 95-100.
Dobre, I.; Georgescu, L.A.; Alexe, P.; Escuredo, O.; Seijo, M.C. Rheological behavior of different honey types from Romania. Food Res. Int. 2012, 49, 126-132. [CrossRef]
Rybac-Chmielewska, H. High performance liquid chromatography (HPLC) study of sugar composition in somekinds of natural honey and winter stores processed by bees from starch syrup. J. Apic. Sci. 2007, 51, 23-38.
Martins, R.C.; Lopes, V.V.; Valentão, P.; Carvalho, J.C.M.F.; Isabel, P.; Amaral, M.T.; Batista, M.T.; Andrade, P.B.; Silva, B.M. Relevant principal component analysis applied to the characterization of Portuguese heather honey. Nat. Prod. Res. 2008, 27, 1560-1582. [CrossRef]
Consonni, R.; Cagliari, L.R.; Cogliati, C. NMR Characterization of saccharides in Italian Honeys of different sources. J. Agric. Food Chem. 2012, 60, 4526-4534. [CrossRef]
Kasperné-Szél, Z. Chemical Characteristics of Milkweed Honey and Its Comparison with Robinia Honey. Ph.D. Thesis, Corvinus University Budapest, Budapest, Hungary, 2006.
Hişil, Y.; Bagdatlio glu, N. Change s in sugars of clear honeys kept in glass jars during storage. Gida Teknol. Dern. Yayin Organi 1994, 19, 103-105.
Goldschmidt, S.; Burkert, H. Uber das Vorkommen im Bienenhonig bisher unbekannter Zucker. Hoppe Seyler Z. Physiol. Chem. 1955, 300, 188-200. [CrossRef]
Belliardo, F.; Buffa, M.; Patetta, A. Manino, A. Identification of melezitose and erlose in floral and honeydew honeys. Carbohyd. Res. 1979, 71, 335-338. [CrossRef]
Fakhari, A.R.; Salehi, P.; Heydari, R.; Ebrahimi, S.N.; Haddad, P.R. Hydrodistillation-headspace solvent microextraction, a new method for analysis of the essential oil components of Lavandula angustifolia Mill. J. Chromatogr. A 2005, 1098, 14-18. [CrossRef]
Shearer, D.A.; Boch, R. 2-heptanone in the mandibular gland secretion of the honey-bee. Nature 1965, 206, 530. [CrossRef]
Bedini, S.; Flamini, G.; Cosci, F.; Ascizzi, R.; Echeverria, M.C.; Guidi, L.; Landi, M.; Lucchi, A.; Conti, B. Artemisia spp. essential oil against the disease-carrying blowfly Calliphora vomitoria. Parasites Vectors 2017, 10, 80. [CrossRef]
Croteau, R. Biosynthesis and catabolism of monoterpenoids. Chem. Rev. 1987, 87, 929-954. [CrossRef]
Dai, J.-P.; Chen, J.; Bei, Y.-F.; Han, B.-X.; Wang, S. Influence of borneol on primary mice oral fibroblasts: A penetration enhancer may be used in oral submucous fibrosis. J. Orally Pathol. Med. 2009, 38, 276-281. [CrossRef]
Rotarescu, R.; Vidican, C. Impact's assessment ofthermal processing and storage conditions onenzimatic activity and HMF content in honey. Carpathian J. Food Sci. Technol. 2010, 2, 1-13.
Pongprayoon, U.; Baeckstrom, P.; Jacobsson, U.; Lindstroem, M.; Bohlin, L. Antispasmodic activity of beta-Damascenone and E-Phytol isolated from Ipomoea pescaprae. Planta Med. 1992, 58, 19-21. [CrossRef]
Castro-Vázquez, L.; Díaz-Maroto, M.C.; Pérez-Coello, M.S. Aroma composition and new chemical markers of Spanish citrus honeys. Food Chem. 2007, 103, 601-606. [CrossRef]
Cuevas-Glory, L.F.; Pino, J.A.; Santiago, L.S.; Sauri-Duch, E. A review of volatile analytical methods for determining the botanical origin of honey. Food Chem. 2007, 103, 1032-1043. [CrossRef]
Jerković, I.; Tuberso, C.I.G.; Gugić, M.; Bubalo, D. Composition of Sulla (Hedysarum coronarium L.) honey solvent extractives determined by GC/MS: Norisoprenoids and other volatile organic compounds. Molecules 2010, 15, 6375-6385. [CrossRef] [PubMed]
Culea, M. Spectrometria de masă. Principii şi aplica¸tii; Risoprint Publishing House: Cluj-Napoca, Romania, 2008; pp. 7-276.
Bastos, C.; Alves, R. Compostos voláteis em méis florais. Quim. Nova 2003, 26, 90-96. [CrossRef]
Gianelli, M.P.; Flores, M.; Toldrá, F. Optimisation of solid phase microextraction (SPME) for the analysis of volatile compounds in dry-cured ham. J. Sci. Food Agric. 2002, 82, 1703-1709. [CrossRef]
Jerkovic, I.; Marijanovic, Z.; Kezic, J.; Gugic, M. Headspace, Volatile and Semi-Volatile Organic Compounds Diversity and Radical Scavenging Activity of Ultrasonic Solvent Extracts from Amorpha fruticosa Honey Samples. Molecules 2009, 14, 2717-2728. [CrossRef]
Radovic, B.S.; Careri, M.; Mangia, A.; Musci, M.; Gerboles, M.; Anklam, E. Contribution of dynamic headspace GC-MS analysis of aroma compounds to authenticity testing of honey. Food Chem. 2001, 72, 511-520. [CrossRef]
Soria, A.C.; Martínez-Castro, I.; Sanz, J. Analysis of volatile composition of honey by solid phase microextraction and gas chromatography-mass spectrometry. J. Sep. Sci. 2003, 26, 793-801. [CrossRef]
Yang, Y.; Battesti, M.J.; Paolini, J.; Muselli, A.; Tomi, P.; Costa, J. Melissopalynological origin determination and volatile composition analysis of Corsican “Erica arborea spring maquis” honeys. Food Chem. 2012, 134, 37-47. [CrossRef]
Ruoff, K. Solid-Phase Microextraction of Honey Volatiles: A Method for the Determination of the Botanical Origin of Honey. Master's Thesis, University of Helsinki, Helsinki, Finland, 2003.
De la Fuente, E.; Martinez-Castro, I.; Sanz, J. Characterization of Spanish unifloral honeys by solid phase microextraction and gas chromatography-mass spectrometry. J. Sep. Sci. 2005, 28, 1093-1100. [CrossRef]
Plutowska, B.; Chmiel, T.; Dymerski, T.; Wardencki, W. A headspace solid-phase microextrasction method development and its application in the determination of volatiles in honeys by gas chromatography. Food Chem. 2011, 126, 1288-1298. [CrossRef]
Bobis, O.; Dezmirean, D.S.; Moise, A. Honey and diabetes: The Importance of Natural Simple Sugars in Diet for Preventing and Treating Different Type of Diabetes. Oxid. Med. Cell. Longev. 2018, 2018, 4757893. [CrossRef]
