Protein elicitor PeaT1 efficiently controlled barley yellow dwarf virus in wheat

Li, Lin; Wang, Shuangchao; Yang, Xiufen; Francis, Frédéric; Qiu, Dewen

doi:10.3390/agriculture9090193

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Protein elicitor PeaT1 efficiently controlled barley yellow dwarf virus in wheat

Li, Lin; Wang, Shuangchao; Yang, Xiufen et al.

2019 • In Agriculture

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/239551

DOI
10.3390/agriculture9090193

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Keywords :

barley yellow dwarf virus; protein elicitor; PeaT1; EPG; virus transmission ability;; virus proliferation

Abstract :

[en] Barley yellow dwarf virus (BYDV), transmitted by the wheat aphid, generates serious wheat yellow dwarf disease and causes great losses in agriculture. Induced resistance has attracted great attention over recent years as a biological method to control plant pathogens and herbivores. Protein elicitor PeaT1 induces defense response in plants against fungi, viruses, and aphids. In this study, wheat seeds and seedlings were soaked and sprayed with 30 g/mL PeaT1, respectively. Then seedlings were inoculated with BYDV by viruliferous Schizaphis graminum to detect the control e ciency of PeaT1-induced resistance against BYDV. The control e ciency was over 30% on the 14th and 21st days after the inoculation access period. Quantitative real time polymerase chain reaction (Q-RT-PCR) tests showed that there was less mRNA from the BYDV coat protein in PeaT1-treated wheat seedlings than in the control group. Electrical penetration graph (EPG) tests showed that virus transmission vector S.graminum took a longer time to find probe and feeding sites on PeaT1-treated wheat seedlings. Additionally, PeaT1-treated wheat seedlings gained higher plant height and more chlorophyll a&b. These results showed that PeaT1 e ciently controlled BYDV by inhibiting BYDV proliferation, reducing the virus transmission ability of S. graminum and alleviating the symptoms of dwarfism and yellow colouring caused by BYDV. This study provided a new integrated way to control BYDV biologically.

Research Center/Unit :

Institute of Plant Protection, Chinese Academy of Agricultural Sciences

Disciplines :

Agriculture & agronomy

Author, co-author :

Li, Lin ; Université de Liège - ULiège > Doct. sc. agro. & ingé. biol. (Paysage)

Wang, Shuangchao

Yang, Xiufen

Francis, Frédéric ; Université de Liège - ULiège > Département GxABT > Gestion durable des bio-agresseurs

Qiu, Dewen

Language :

English

Title :

Protein elicitor PeaT1 efficiently controlled barley yellow dwarf virus in wheat

Publication date :

06 September 2019

Journal title :

Agriculture

eISSN :

2077-0472

Publisher :

MDPI AG, Basel, Switzerland

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

National Key R&D Program of China

Available on ORBi :

since 19 September 2019

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