Article (Scientific journals)
Precise co-registration of mass spectrometry imaging, histology, and laser microdissection-based omics
Dewez, Frédéric; Martin-Lorenzo, Marta; Herfs, Michael et al.
2019In Analytical and Bioanalytical Chemistry, 411, p. 5647-5653
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Keywords :
Mass spectrometry imaging; laser microdissection; microproteomics; co-registration; intra-tumor heterogeneity
Abstract :
[en] Mass spectrometry imaging (MSI) is an analytical technique for the unlabeled and multiplex imaging of molecules in biological tissue sections. It therefore enables the spatial and molecular annotation of tissues complementary to histology. It has already been shown that MSI can guide subsequent material isolation technologies such as laser microdissection (LMD) to enable a more in-depth molecular characterization of MSI-highlighted tissue regions. However, with MSI now reaching spatial resolutions at the single-cell scale, there is a need for a precise co-registration between MSI and the LMD. As proof-of-principle, MSI was performed on a breast cancer tissue and segmentation on the lipid data was performed to detect molecularly distinct segments within its tumor areas. After image processing of the segmentation results, the coordinates of the MSI detected segments were passed to the LMD system by three co-registration steps. The errors of each co-registration step were quantified and the total error was found to be less than 13 micrometers. With this link established, MSI data can now accurately guide LMD to excise MSI-defined regions of interest for subsequent extract-based analyses. In our example, the excised tissue material was then subjected to ultrasensitive microproteomics in order to determine predominant molecular mechanisms in each of the MSI highlighted intratumor segments. This work shows how the strengths of MSI, histology, and extract-based omics can be combined to enable a more comprehensive molecular characterization of in situ biological processes.
Disciplines :
Author, co-author :
Dewez, Frédéric ;  Université de Liège - ULiège > Form. doct. sc. (chimie - paysage)
Martin-Lorenzo, Marta
Herfs, Michael ;  Université de Liège - ULiège > Cancer-Experimental Pathology
Baiwir, Dominique  ;  Université de Liège - ULiège > GIGA Platforms
Mazzucchelli, Gabriel  ;  Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
De Pauw, Edwin  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Heeren, Ron M.A
Balluff, Benjamin
Language :
Title :
Precise co-registration of mass spectrometry imaging, histology, and laser microdissection-based omics
Alternative titles :
[en] France
Publication date :
14 June 2019
Journal title :
Analytical and Bioanalytical Chemistry
Publisher :
Springer, Germany
Volume :
Pages :
Peer reviewed :
Peer Reviewed verified by ORBi
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