Linking CRISPR-Cas9 interference in cassava to the evolution of editing-resistant geminiviruses.

[en] BACKGROUND: Geminiviruses cause damaging diseases in several important crop species. However, limited progress has been made in developing crop varieties resistant to these highly diverse DNA viruses. Recently, the bacterial CRISPR/Cas9 system has been transferred to plants to target and confer immunity to geminiviruses. In this study, we use CRISPR-Cas9 interference in the staple food crop cassava with the aim of engineering resistance to African cassava mosaic virus, a member of a widespread and important family (Geminiviridae) of plant-pathogenic DNA viruses. RESULTS: Our results show that the CRISPR system fails to confer effective resistance to the virus during glasshouse inoculations. Further, we find that between 33 and 48% of edited virus genomes evolve a conserved single-nucleotide mutation that confers resistance to CRISPR-Cas9 cleavage. We also find that in the model plant Nicotiana benthamiana the replication of the novel, mutant virus is dependent on the presence of the wild-type virus. CONCLUSIONS: Our study highlights the risks associated with CRISPR-Cas9 virus immunity in eukaryotes given that the mutagenic nature of the system generates viral escapes in a short time period. Our in-depth analysis of virus populations also represents a template for future studies analyzing virus escape from anti-viral CRISPR transgenics. This is especially important for informing regulation of such actively mutagenic applications of CRISPR-Cas9 technology in agriculture.

Disciplines :

Biotechnology

Author, co-author :

Mehta, Devang

Sturchler, Alessandra

Anjanappa, Ravi B.

Syed-Shan-e-Ali, Zaïdi ; Université de Liège - ULiège > Département GxABT > Plant Sciences

Hirsch-Hoffmann, Matthias

Gruissem, Wilhelm

Vanderschuren, Hervé ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Ingénierie des productions végétales et valorisation

Language :

English

Title :

Linking CRISPR-Cas9 interference in cassava to the evolution of editing-resistant geminiviruses.

Publication date :

25 April 2019

Journal title :

Genome Biology

ISSN :

1474-7596

eISSN :

1474-760X

Publisher :

Springer, London, United Kingdom

Volume :

Issue :

Pages :

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 29 June 2019

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Scopus citations^®

148

Scopus citations^®
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144

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164

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