New sensitive and fast detection of Little cherry virus 1 using loop-mediated isothermal amplification (RT-LAMP)

[en] Little cherry virus 1 (LChV-1) belongs to the genus Velarivirus, family Closteroviridae, is an economically important pathogen affecting mainly cherry around the world emphasizing the impetus for its efficient and accurate on-site detection. This study describes the development of a reliable diagnostic protocol of LChV-1 based on a one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP). The protocol detects LChV-1 isolates in less than 10 min by fluorescence monitoring using a mobile detection device and is most optimal when performed at 67 °C. Sharp melting curves and unique melting temperatures (Tm) were obtained for the positive samples. Both the RT-LAMP and classical RT-PCR methods are capable of specifically detecting LChV-1 in infected leaf tissues. In addition, the RT-LAMP has remarkable advantages in comparison to RT-PCR. It is at least hundred fold more sensitive, significantly faster (allowing on-field leaf-to-result diagnostic) and efficient at minimal cost. In conclusion, this innovative RT-LAMP approach can contribute to the implementation of sustainable integrated management strategies for detection of LChV-1 in commercial orchards or for horticultural research stations. It is also suitable for decision support in phytosanitary epidemiological programs. © 2019 Elsevier B.V.

Disciplines :

Phytobiology (plant sciences, forestry, mycology...)

Author, co-author :

Tahzima, Rachid ; Plant Sciences Unit, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Merelbeke, 9820, Belgium, Laboratory of Integrated and Urban Phytopathology, University of Liège (ULg), Gembloux Agro-Bio tech, Gembloux, 5030, Belgium

Foucart, Y.; Plant Sciences Unit, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Merelbeke, 9820, Belgium

Peusens, G.; Department of Zoology, Proefcentrum Fruitteelt (pcfruit), Sint-Truiden, 3800, Belgium

Beliën, T.; Department of Zoology, Proefcentrum Fruitteelt (pcfruit), Sint-Truiden, 3800, Belgium

Massart, Sébastien ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Gestion durable des bio-agresseurs

De Jonghe, K.; Plant Sciences Unit, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Merelbeke, 9820, Belgium

Language :

English

Title :

New sensitive and fast detection of Little cherry virus 1 using loop-mediated isothermal amplification (RT-LAMP)

Publication date :

2019

Journal title :

Journal of Virological Methods

ISSN :

0166-0934

eISSN :

1879-0984

Publisher :

Elsevier B.V.

Volume :

265

Pages :

91-98

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 June 2019

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