Article (Scientific journals)
Skeletal muscle ceramides do not contribute to physical inactivity-induced insulin resistance.
Appriou, Zephyra; Nay, Kevin; Pierre, Nicolas et al.
2019In Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme
Peer reviewed
 

Files


Full Text
skeletal muscle ceramides do not contribute.pdf
Publisher postprint (853.82 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] Physical inactivity increases the risk to develop type 2 diabetes, a disease characterized by a state of insulin resistance. By promoting inflammatory state, ceramides are especially recognized to alter insulin sensitivity in skeletal muscle. The present study was designed to analyze, in mice, whether muscle ceramides contribute to physical inactivity-induced insulin resistance. For this purpose, we used the wheel lock model to induce a sudden reduction of physical activity, in combination with myriocin treatment, an inhibitor of de novo ceramide synthesis. Mice were assigned to 3 experimental groups: voluntary wheel access group (Active), a wheel lock group (Inactive) and wheel lock group treated with myriocin (Inactive-Myr). We observed that 10 days of physical inactivity induces hyperinsulinemia and increases HOMA-IR. The muscle ceramide content was not modified by physical inactivity and myriocin. Thus, muscle ceramides do not play a role in physical inactivity-induced insulin resistance. In skeletal muscle, insulin-stimulated Akt phosphorylation and inflammatory pathway were not affected by physical inactivity whereas a reduction of GLUT4 content was observed. Based on these results, physical inactivity-induced insulin resistance seems related to a reduction in GLUT4 content rather than defects in insulin signaling. We observed in inactive mice that myriocin treatment improves glucose tolerance, insulin-stimulated Akt, AMPK activation and GLUT4 content in skeletal muscle. Such effects occur regardless of changes in muscle ceramide content. These findings open promising research perspectives to identify new mechanisms of action for myriocin on insulin sensitivity and glucose metabolism.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Appriou, Zephyra
Nay, Kevin
Pierre, Nicolas  ;  Université de Liège - ULiège > I3-Translational gastroenterology
Saligaut, Dany
Lefeuvre-Orfila, Luz
Martin, Brice
Cavey, Thibault
Ropert, Martine
Loreal, Olivier
Rannou-Bekono, Francoise
Derbre, Frederic
Language :
English
Title :
Skeletal muscle ceramides do not contribute to physical inactivity-induced insulin resistance.
Publication date :
2019
Journal title :
Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme
ISSN :
1715-5312
eISSN :
1715-5320
Peer reviewed :
Peer reviewed
Available on ORBi :
since 19 June 2019

Statistics


Number of views
39 (2 by ULiège)
Number of downloads
1 (1 by ULiège)

Scopus citations®
 
10
Scopus citations®
without self-citations
10
OpenCitations
 
9

Bibliography


Similar publications



Contact ORBi