Article (Scientific journals)
Aging related ER stress is not responsible for anabolic resistance in mouse skeletal muscle.
Chalil, Sreeda; Pierre, Nicolas; Bakker, Astrid D. et al.
2015In Biochemical and Biophysical Research Communications, 468 (4), p. 702-7
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Keywords :
Aging/physiology; Animals; Endoplasmic Reticulum/physiology; Energy Metabolism/physiology; Male; Mice; Mice, Inbred C57BL; Muscle Proteins/biosynthesis; Muscle, Skeletal/physiology; Organ Size/physiology; Stress, Physiological/physiology; Leucine; S6K1; Sarcopenia; Tunicamycin; Unfolded protein response
Abstract :
[en] Anabolic resistance reflects the inability of skeletal muscle to maintain protein mass by appropriate stimulation of protein synthesis. We hypothesized that endoplasmic reticulum (ER) stress contributes to anabolic resistance in skeletal muscle with aging. Muscles were isolated from adult (8 mo) and old (26 mo) mice and weighed. ER stress markers in each muscle were quantified, and the anabolic response to leucine was assessed by measuring the phosphorylation state of S6K1 in soleus and EDL using an ex vivo muscle model. Aging reduced the muscle-to-body weight ratio in soleus, gastrocnemius, and plantaris, but not in EDL and tibialis anterior. Compared to adult mice, the expression of ER stress markers BiP and IRE1alpha was higher in EDL, and phospho-eIF2alpha was higher in soleus and EDL of old mice. S6K1 response to leucine was impaired in soleus, but not in EDL, suggesting that anabolic resistance contributes to soleus weight loss in old mice. Pre-incubation with ER stress inducer tunicamycin before leucine stimulation increased S6K1 phosphorylation beyond the level reached by leucine alone. Since tunicamycin did not impair leucine-induced S6K1 response, and based on the different ER stress marker regulation patterns, ER stress is probably not involved in anabolic resistance in skeletal muscle with aging.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Chalil, Sreeda
Pierre, Nicolas  ;  Université de Liège - ULiège > Département des sciences cliniques > Hépato-gastroentérologie
Bakker, Astrid D.
Manders, Ralph J.
Pletsers, Annelies
Francaux, Marc
Klein-Nulend, Jenneke
Jaspers, Richard T.
Deldicque, Louise
Language :
English
Title :
Aging related ER stress is not responsible for anabolic resistance in mouse skeletal muscle.
Publication date :
2015
Journal title :
Biochemical and Biophysical Research Communications
ISSN :
0006-291X
eISSN :
1090-2104
Publisher :
Elsevier, Atlanta, United States - California
Volume :
468
Issue :
4
Pages :
702-7
Peer reviewed :
Peer reviewed
Commentary :
Copyright (c) 2015 Elsevier Inc. All rights reserved.
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since 18 June 2019

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