Article (Scientific journals)
ELISA detection of bovine viral diarrhoea virus specific antibodies using recombinant antigen and monoclonal antibodies.
Lecomte, C.; Pin, J. J.; De Moerlooze, L. et al.
1990In Veterinary Microbiology, 23 (1-4), p. 193-201
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Keywords :
Animals; Antibodies, Monoclonal/immunology; Antibodies, Viral/analysis; Antibody Specificity; Antigens, Viral/immunology; Binding, Competitive; Cattle; Diarrhea Viruses, Bovine Viral/immunology; Enzyme-Linked Immunosorbent Assay; Fluorescent Antibody Technique; Hybridomas; Mice; Mice, Inbred BALB C; Neutralization Tests; Pestivirus/immunology; Radioimmunoprecipitation Assay; Recombinant Proteins/immunology
Abstract :
[en] A panel of monoclonal antibodies was prepared by immunization of BALB/c mice with Moredun (BD) virus strains. These antibodies were characterized by immunofluorescence and seroneutralization against BD, BVD and hog cholera (HC) virus strains, and radioimmunoprecipitation of BVD-infected cells extracts. The MAbs reacting with the majority of the Pestivirus strains recognize the 80 kDa antigen of the BVD cytophathic strains. The 80 kDa antigen of the BVD/Osloss virus strain has been cloned and expressed in E. coli as a fusion protein with beta-galactosidase. The fusion protein has been purified from inclusion bodies and used successfully as an antigen for ELISA detection of BVDV specific antibodies in bovine sera. A competitive ELISA using MAbs is more specific than a direct assay. These results compare well with the ones obtained with antigen extracted from BVDV-infected cells.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Lecomte, C.;  Eurogentec (Liège)
Pin, J. J.;  Rhône-Mérieux (Lyon)
De Moerlooze, L.;  Eurogentec (Liège)
Vandenbergh, D.;  Eurogentec (Liège)
Lambert, A.F.;  Eurogentec (Liège)
Pastoret, Paul-Pierre ;  Université de Liège - ULiège > Faculté de Médecine vétérinaire
Chappuis, G.;  Rhône-Mérieux, laboratoires IFFA, Lyon
Language :
English
Title :
ELISA detection of bovine viral diarrhoea virus specific antibodies using recombinant antigen and monoclonal antibodies.
Publication date :
1990
Journal title :
Veterinary Microbiology
ISSN :
0378-1135
eISSN :
1873-2542
Publisher :
Elsevier, Netherlands
Volume :
23
Issue :
1-4
Pages :
193-201
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 01 June 2019

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