Keywords :
Animals; Antibodies, Monoclonal/immunology; Antibodies, Viral/analysis; Antibody Specificity; Antigens, Viral/immunology; Binding, Competitive; Cattle; Diarrhea Viruses, Bovine Viral/immunology; Enzyme-Linked Immunosorbent Assay; Fluorescent Antibody Technique; Hybridomas; Mice; Mice, Inbred BALB C; Neutralization Tests; Pestivirus/immunology; Radioimmunoprecipitation Assay; Recombinant Proteins/immunology
Abstract :
[en] A panel of monoclonal antibodies was prepared by immunization of BALB/c mice with Moredun (BD) virus strains. These antibodies were characterized by immunofluorescence and seroneutralization against BD, BVD and hog cholera (HC) virus strains, and radioimmunoprecipitation of BVD-infected cells extracts. The MAbs reacting with the majority of the Pestivirus strains recognize the 80 kDa antigen of the BVD cytophathic strains. The 80 kDa antigen of the BVD/Osloss virus strain has been cloned and expressed in E. coli as a fusion protein with beta-galactosidase. The fusion protein has been purified from inclusion bodies and used successfully as an antigen for ELISA detection of BVDV specific antibodies in bovine sera. A competitive ELISA using MAbs is more specific than a direct assay. These results compare well with the ones obtained with antigen extracted from BVDV-infected cells.
Lecomte, C.; Eurogentec (Liège)
Pin, J. J.; Rhône-Mérieux (Lyon)
De Moerlooze, L.; Eurogentec (Liège)
Vandenbergh, D.; Eurogentec (Liège)
Lambert, A.F.; Eurogentec (Liège)
Chappuis, G.; Rhône-Mérieux, laboratoires IFFA, Lyon
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