E. coli O157; gene encoding the virulence factors; ovine carcasses; swabbing; Algiers City Slaughterhouses
Abstract :
[en] Objective: This study aims to identify and characterize Escherichia coli O157 strains isolated from ovine carcasses of two slaughterhouses of Algiers city. Material and method: One hundred and fifty-one (151) parts of sheep carcasses were swabbed by a non-destructive method based on double swabbing process (wet/dry) at two slaughterhouses of Algiers city for the identification and investigation of E. coli O157 strains. The E. coli O157: H7 isolation required a non-selective enrichment step followed by one of immuno-concentration of the bacteria throughout the immunomagnetic separation (IMS) technique and another one for bacteria isolation (on CT-SMAC agar - Cefixime Tellurite Sorbitol–MacConkey). The presence of O157 somatic antigen and H7 flagellar antigen in the isolated suspicious doubtful colonies was confirmed by the anti-O157 latex and H7 antiserum agglutination assays. Confirmation of the virulence of the isolated E. coli O157: H7 strain was obtained performed by gene amplification (PCR – Polymerase Chain Reaction). Results: The presence of E. coli O157: H7 was shown in Eleven ovine carcasses out of the 151 tested with a prevalence rate of 7.26%. Nine of these carcasses came from the first laughterhouse and two of them from the second one. Amongst the eleven positive carcasses, thirteen strains of E. coli O157: H7 were isolated: ten strains are sorbitol and β-glucuronidase-negative, and three of them are sorbitol and β-glucuronidase-positive. Among them, seven strains (53.85%) showed an
eae stx2 pathotype, one strain (7.69%) showed eae stx1 pathotype, one strain (7.69%) showed the eae pathotype, and three strains (23.08%) showed stx2 pathotype. Only one strain (7.69%) had no gene encoding the virulence factors. Conclusion: The obtained results revealed that
sheep are carriers of E. coli O157: H7 in Algeria. Appropriate hygiene and control measures at the slaughterhouse level must be implemented to provide a safe product for the consumer.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Ferhat, L.
Chahed, Amina; National Higher Veterinary School of Algiers
China, Bernard; Belgian Scientific Institute for Public Health
Assaous, Farida; Pasteur Institute, Dely Ibrahim, Algeria
Daube, Georges ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Rahal; Pasteur Institute, Dely Ibrahim, Algeria
Language :
English
Title :
Research and characterization of Escherichia coli O157 strains isolated from ovine carcasses of two slaughterhouses of Algiers city
AFSSA. The opinion of 27 May 2010 on the relevance of the definition of pathogenic STECs, specified by Afssa opinion (Opinion of 27 May 2010 on the relevance of the pathogenic definition of STECs, specified by Afssa opinion). Maisons-Alfort; 2010: 19
Ammon A, Petersen LR, Karch H. A large outbreak of hemolytic uremic syndrome caused by unusual sorbitol-fermenting strain of Escherichia coli O157. J Infec Dis 1999;179:1274-1277
Armstrong GL, Hollingsworth J, Morris JG. Emerging foodborne pathogens: Escherichia coli O157: H7 as model of entry of a new pathogen into the food supply of the developed world. Epidemiol Rev 1996;18:29-51
Beutin L, Geier D, Steinruck H, Zimmermann S, Scheutz F. Prevalence and some properties of verotoxin (Shiga-like toxin)-producing Escherichia coli in seven different species of healthy domestic animals. J Clin Microbiol 1993;31(9):2483-2488
Beutin L, Geier D, Zimmermann S, Karch H. Virulence markers of Shiga-like toxin-producing Escherichia coli strains originating from healthy domestic animals of different species. J Clin Microbiol 1995;33:631-5
Blanco J, Blanco M, Blanco JE, Mora A, Gonzalez A, Bernardez MI, et al. Verotoxin-Producing Escherichia coli in Spain: Prevalence, serotypes, and virulence genes of O157 :H7 and non-O157 VTEC in ruminants, raw beef products, and humans. Exper Biol Med 2003;228:345-351
Caprioli A, Morabito S, Brugere H, Oswald E. Enterohaemorrhagic Escherichia coli: emerging issues on virulence and modes of transmission. Vet Res 2005;36:289-311
Chahed A, China B, Mainil J, Daube G. Prevalence of enterohaemorragic Escherichia coli from serotype O157 and other attaching and effacing Escherichia coli on bovine carcasses in Algeria. J Appl Microbiol 2006;101:361-368
Chapman PA, Cornell J, Green C. Infection with verocytotoxin-producing Escherichia coli O157 during a visit to an inner city open farm. Epidemiol Infec 2000;125:531-6
Chapman PA, Ashton R. An evaluation of rapid methods for detecting Escherichia coli O157 on beef carcasses. Inter J Food Microbiol 2003;87:279-285
China B, Prison V, Mainil J. Typing of bovine attaching and effacing Escherichia coli by multiplex in vitro amplification of virulenceassociated genes. Appl Environ Microbiol 1996;62:3462-3465
Espié E, Vaillant V. Toxi-infection alimentaire collective à Escherichia coli O148 :H8 producteur de shigatoxines (Collective food-borne infections to Escherichia coli O148: H8 producing shigatoxins). Rapport d'investigation 2002;44
Fegan N, Desmarchelier P. Shiga toxin-producing Escherichia coli in sheep and pre-slaughter lambs in Eastern Australia. Lett Appl Microbiol 1999;28(5):335-9
Griffin PM, Tauxe RV. The epidemiology of infections caused by Escherichia coli O157: H7, other Enterohemorrhagic Escherichia coli and the associated hemolytic uremic syndrome. Epidemiol Rev 1991;13:60-92
Heuvelink AE, Van Den Biggelaar FL, de Boer E, Herbes RG, Melchers WJ, Huisin't Veld JH, Monnens LA. Isolation and characterization of verocytotoxin-producing Escherichia coli O157 strains from Dutch cattle and sheep. J Clin Microbiol 1998;36:878-882
ISO 17604, Microbiology of food and animal feeding stuffs-Sampling of carcasses for microbiological analysis. 2003
Joly B, Reynaud A. Enterobacteriaceae Systematic and diagnostic methods (Enterobacteriaceae Systematic and diagnostic methods). Coll. Microbiology Monographs 2003. (2th ed.). TEC & DOC, 356p
Karch H. Origin of EHEC. Abstracts of the second international symposium of the European study group on Enterohemorrhagic Escherichia coli. Acta Clin Belg 1999;54(1):48
Khudor MH, Issa AH, Jassim FL. Detection of rfb O157 and fliC H7 genes in Escherichia coli isolated from humen and sheep in Basrah Province. Raf J Sci 2012;23:19-33
Koch C, Hertwig S, Lurz R, Appel B, Beutin L. Isolation of a lysogenic bacteriophage carrying the stx OX3 gene which is closely associated with Shiga toxin-producing Escherichia coli strains from sheep and humans. J Clin Microbiol 2001;39:3992-3998
Kudva IT, Hatfield PG, Hovde CJ. Escherichia coli O157:H7 in microbial flora of sheep. J Clin Microbiol 1996;34:431-433
Phillips D, Jordan D, Morris S, Jenson I, Summner J. Microbiological quality of Australian sheep meat in 2004. Meat Science 2006;74:261-266
Riley LW, Remis RS, Helgerson SD, Mcgee HB, Wells JG, Davis BR, et al. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N Engl J Med 1983;308:681-685
Seibt F, Filer G, Gellermann J, Beutin L, Ehrih JHH. The heterogeneity of haemolytic uraemic syndromes in children and adults. Nephrologisches jahresgespräch 1995; Editor: Deutsche Dialysegesellschaft. IN TEXT IS 1997
Söderlund R, Hedenström I, Nilsson A, Eriksson A, Aspán A. Genetical lysimilar strains of Escherichia coli O157:H7 isolated from sheep, cattle andhuman patients. BMC Vet Res 2012;8:200
Vettorato MP, Leomil L, Guth BEC, Irino K, Pestana De Castro AF. Properties of Shiga toxin-producing Escherichia (STEC) isolates from sheep in the State of Sao Paulo, Brazil Vet Microbiol 2003;95:103-109
Zarei M, Basiri N, Jamnejad A, Eskandari MH. Prevalance of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella SPP. In Beef, Buffalo and lamb using multiplex PC. Jundishapur Microbiol 2013;6(8):7244
Zweifel C, Stephan R. Microbiological monitoring of sheep carcass contamination in three Swiss abattoirs. J. Food Port 2003;66:946-952
Zweifel C, Blanco JE, Blanco M, Blanco J, Stephan R. Serotypes and virulence genes of ovine non-O157 Shiga toxin-producing Escherichia (STEC) from Swiss cattle.Vet Microbiol 2005;105:37-47
Zweifel C, Kaufman M, Blanco J, Stephan R. Significance of Escherichia coli O157:H7 in sheep at slaughter in Switzerland. Schwerz Arch Tierheilkd 2006;148 (6):289-95
