Quantitative phosphoproteomic analysis of ovine muscle with different postmortem glycolytic rates

Glycolysis; Glycolytic rate; Muscle contraction; Phosphoproteomic; Protein phosphorylation; Cell signaling; Enzymes; Pathology; Phosphorylation; Muscle contractions; Muscle; Article

Abstract :

[en] Phosphorylation regulates protein structure, function, cell signaling and enzyme activities within cells. Postmortem changes of muscle to meat are partially determined by the structure, function and enzyme activities of proteins. To further understand the mechanisms regulating postmortem changes, ovine muscles with different glycolytic rates were subjected to quantitative phosphoproteomic analysis. Totally 116 unique phosphopeptides matched to 99 phosphoproteins were detected to be different in abundance among the fast, moderate and slow glycolytic rate muscles. Of which, 24 phosphoproteins clustered into glycolysis and muscle contraction were selected after bioinformatics analysis. Quantitative analysis showed that phosphorylation of pyruvate kinase, phosphoglucomutase 1, enolase and fructose-bisphosphate aldolase was correlated with glycolytic rate early postmortem. In addition, some myofibrillar proteins were detected to be differentially phosphorylated. In summary, this study revealed that protein phosphorylation at early postmortem may indirectly affect the glycolysis pathway through the regulation of proteins involved in glycolysis and muscle contraction. © 2018

Disciplines :

Animal production & animal husbandry

Author, co-author :

Chen, Li ; Université de Liège - ULiège > Doct. sc. agro. & ingé. biol. (Paysage)

Li, Z.; Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing, 100193, China

Everaert, Nadia ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Ingénierie des productions animales et nutrition

Lametsch, R.; Department of Food Science, Faculty of Science, University of Copenhagen, Frederiksberg, DK-1958, Denmark

Zhang, D.; Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing, 100193, China

Language :

English

Title :

Quantitative phosphoproteomic analysis of ovine muscle with different postmortem glycolytic rates

Publication date :

2019

Journal title :

Food Chemistry

ISSN :

0308-8146

eISSN :

1873-7072

Publisher :

Elsevier Ltd

Volume :

280

Pages :

203-209

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 08 May 2019

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