Article (Scientific journals)
Epoxiconazole exposure affects terpenoid profiles of oilseed rape plantlets based on a targeted metabolomic approach
Durenne, Bastien; Blondel, Alodie; Druart, Philippe et al.
2019In Environmental Science and Pollution Research, 26, p. 17362–17372
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Keywords :
Epoxiconazole; Oilseed rape; VOCs; Metabolic markers; Terpenes; Sulphur-containing volatiles
Abstract :
[en] Epoxiconazole is a broad-spectrum fungicide described as highly persistent in soil and as such can be considered as an abiotic agent like other problematic agrochemicals. Furthermore, the plant phenotyping tool involving non-invasive monitoring of plant-emitted volatile organic compounds (VOCs) may be useful in the identification of metabolic markers for abiotic stress. We therefore decided to profile the VOCs from secondary metabolism of oilseed rape through a dose-response experiment under several epoxiconazole concentrations (0, 0.01, 0.1 and 1 mg L−1). VOC collections of 35-day-old whole plantlets were performed through a dynamic headspace sampling technique under defined and controlled conditions. The plantlets grew freely within a home-made, laboratory and high-throughput glass chamber without any disturbance. Putative metabolic markers were analysed using a targeted metabolomic approach based on TD-GC-MS method coupled with data acquisition in SIM mode in order to focus on terpenes and sulphur-containing volatiles. Chromatograms of emitted terpenes were achieved accurately for the 35-day-old oilseed rape plantlets. We also analysed the presence of sulphur-containing volatiles in samples of shoot and root tissues using an innovative DHS-TD-GC-MS method, but no difference was found between qualitative profiles. Nevertheless, we demonstrated through this experiment that sesquiterpenes such as β-elemene and (E,E)-α-farnesene are involved in epoxiconazole dose-response. In particular, (E,E)-α-farnesene could serve as a metabolic marker of fungicide exposure for oilseed rape plantlets.
Research center :
Centre wallon de recherches agronomiques
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Durenne, Bastien ;  Université de Liège - ULiège > Doct. sc. agro. & ingé. biol. (Paysage)
Blondel, Alodie;  CRA-W
Druart, Philippe;  CRA-W
Fauconnier, Marie-Laure  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
Language :
English
Title :
Epoxiconazole exposure affects terpenoid profiles of oilseed rape plantlets based on a targeted metabolomic approach
Publication date :
April 2019
Journal title :
Environmental Science and Pollution Research
ISSN :
0944-1344
eISSN :
1614-7499
Publisher :
Springer, Germany
Volume :
26
Pages :
17362–17372
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 April 2019

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